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Mouse brain LC-MS - KIBRA controls exosome secretion via inhibiting the proteasomal degradation of Rab27a

ABSTRACT: To investigate the molecular mechanisms by which KIBRA regulates exosome secretion, we performed mass spectral (MS) analysis and an isobaric tag for relative and absolute quantitation (iTRAQ) assay to screen the differentially-expressed proteins in the brains of KIBRA-KO mice compared with their WT littermates.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Brain

SUBMITTER: Lin Song

LAB HEAD: Yifeng Du

PROVIDER: PXD012430 | Pride | 2019-04-02

REPOSITORIES: Pride

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KIBRA controls exosome secretion via inhibiting the proteasomal degradation of Rab27a.

Song Lin L Tang Shi S Han Xiaolei X Jiang Ziying Z Dong Lingling L Liu Cuicui C Liang Xiaoyan X Dong Jixin J Qiu Chengxuan C Wang Yongxiang Y Du Yifeng Y

Nature communications 20190409 1

Exosomes are nanosized membrane vesicles released from cells after fusion of multivesicular bodies (MVBs) with the plasma membrane (PM) and play important roles in intercellular communication and numerous biological processes. However, the molecular mechanisms regulating exosome secretion remain poorly understood. Here we identify KIBRA as an adaptor-like protein that stabilizes Rab27a, which in turn controls exosome secretion both in vitro and in vivo. Knockdown or overexpression of KIBRA in ne ...[more]

PMID: 30967557

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