Dataset Information

PREDICTIVE MODELING OF THERAPEUTIC RESPONSE TO CHONDROITIN SULFATE/GLUCOSAMINE HYDROCHLORIDE IN KNEE OSTEOARTHRITIS

ABSTRACT: In the present study we explored potential protein biomarkers useful to predict the therapeutic response of osteoarthritis (OA) patients treated with pharmaceutical grade Chondroitin sulfate/Glucosamine hydrochloride (CS+GH; Droglican, Bioiberica), in order to optimize therapeutic outcomes.

INSTRUMENT(S): 4800 Proteomics Analyzer

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Serum

SUBMITTER: valentina calamia

LAB HEAD: Francisco J Blanco Garcia

PROVIDER: PXD012444 | Pride | 2019-09-12

REPOSITORIES: Pride

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Publications

Predictive modeling of therapeutic response to chondroitin sulfate/glucosamine hydrochloride in knee osteoarthritis.

Blanco Francisco J FJ Camacho-Encina María M González-Rodríguez Lucía L Rego-Pérez Ignacio I Mateos Jesús J Fernández-Puente Patricia P Lourido Lucía L Rocha Beatriz B Picchi Florencia F Silva-Díaz María T MT Herrero Marta M Martínez Helena H Verges Josep J Ruiz-Romero Cristina C Calamia Valentina V

Therapeutic advances in chronic disease 20190824

<h4>Background</h4>In the present study, we explored potential protein biomarkers useful to predict the therapeutic response of knee osteoarthritis (KOA) patients treated with pharmaceutical grade Chondroitin sulfate/Glucosamine hydrochloride (CS+GH; Droglican, Bioiberica), in order to optimize therapeutic outcomes.<h4>Methods</h4>A shotgun proteomic analysis by iTRAQ labelling and liquid chromatography-mass spectrometry (LC-MS/MS) was performed using sera from 40 patients enrolled in the Multic ...[more]

PMID: 31489155

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Project description:It has been recognised for a long time that cell surface glycans plays a vital role in the biological process and their altered form can lead to cancer. However, the molecular details and regulatory mechanism between the glycans and cancer is yet not clear. Over the past decade mass spectrometry-based techniques has become a prominent method for analysing glycans especially N-glycan matrix assisted laser desorption/ionisation mass spectrometry imaging (MALDI MSI). It is a powerful technique that combines mass spectrometry with histology, enabling the visualisation and label free detection of N-linked glycans on a single tissue section. Here, we carried out N-glycan MALDI MSI on six endometrial cancer (EC) formalin fixed paraffin embedded (FFPE) tissue sections including the adjacent normal endometrium, and tissue microarrays (TMA) consisting of 8 EC patients with lymph node metastasis (LNM) and 20 without LNM. By doing that several m/z values were detected that can significantly distinguish normal endometrium from cancerous. Also, detected a m/z value that can discriminate the primary tumour with LNM from those without. Identification of those discriminative m/z values was performed using porous graphitized carbon liquid chromatography tandem mass spectrometry (PGC-LC-MS/MS). Overall, we observed higher abundance of oligomannose in tumour regions compared to normal with AUC ranges from 0.85-0.99. Whereas, complex N-glycans were detected in lower abundance with AUC ranges from 0.03-0.28. Comparison of N-glycans between the primary tumours with LNM and without LNM indicates reduced abundance of a complex core-fucosylated N-glycan in patients with metastasis relative to without. In summary, N-glycan MALDI MSI can be used to characterize the cancerous endometrium from the normal, also patients with LNM from those without. Identification of those discriminative m/z values was performed using porous graphitized carbon liquid chromatography tandem mass spectrometry (PGC-LC-MS/MS).

Dataset Information

PREDICTIVE MODELING OF THERAPEUTIC RESPONSE TO CHONDROITIN SULFATE/GLUCOSAMINE HYDROCHLORIDE IN KNEE OSTEOARTHRITIS

Publications

Predictive modeling of therapeutic response to chondroitin sulfate/glucosamine hydrochloride in knee osteoarthritis.

Similar Datasets

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