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Plasma Proteome Dynamics in the Pig Model of Trauma

ABSTRACT: The effect of IV-administered Vitamin C on the plasma proteome in the pig trauma model was investigated with label free proteomics. Plasma samples were drawn from 9 male Sinclair swine at baseline, post-trauma, and 0.25, 2, and 4 hours post resuscitation with 500 mL of hydroxyethyl starch. Swine were randomized to receive either saline, low-dose VitC (50 mg/kg), or high-dose VitC (200 mg/kg) during volume resuscitation (N = 3/treatment). High performance LC-MS/MS proteomics was then used to quantitatively measure plasma protein levels over time.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Sus Scrofa Domesticus (domestic Pig)

TISSUE(S): Blood Plasma

DISEASE(S): Wounds And Injuries

SUBMITTER: Adam Hawkridge

LAB HEAD: Adam Hawkridge

PROVIDER: PXD012454 | Pride | 2019-07-29

REPOSITORIES: Pride

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Temporal map of the pig polytrauma plasma proteome with fluid resuscitation and intravenous vitamin C treatment.

Cudjoe Emmanuel K EK Hassan Zaneera H ZH Kang Le L Reynolds Penny S PS Fisher Bernard J BJ McCarter Jacquelyn J Sweeney Christopher C Martin Erika J EJ Middleton Paul P Ellenberg Matthew M Fowler Alpha A AA Spiess Bruce D BD Brophy Donald F DF Hawkridge Adam M AM Natarajan Ramesh R

Journal of thrombosis and haemostasis : JTH 20190825 11

<h4>Background</h4>Fluid resuscitation plays a prominent role in stabilizing trauma patients with hemorrhagic shock yet there remains uncertainty with regard to optimal administration time, volume, and fluid composition (e.g., whole blood, component, colloids) leading to complications such as trauma-induced coagulopathies (TIC), acidosis, and poor oxygen transport. Synthetic fluids in combination with antioxidants (e.g., vitamin C) may resolve some of these problems.<h4>Objectives</h4>We applied ...[more]

PMID: 31322812

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