Dataset Information

Carbo-loading in fungal lung pathogens: a quantitative analysis of CAZyme abundance and resulting glycan populations

ABSTRACT: Herein we i) identify Coccidioides spp.-specific CAZymes by bioinformatically comparing the CAZyme repertoire (CAZome) of Coccidioides spp. to other common fungal lung pathogens and a non-pathogenic close fungal relative, ii) experimentally evaluate Coccidioides spp. CAZyme abundance in vivo and in vitro, and iii) identify Coccidioides genus-specific N-glycans by experimentally determining the N-glycan population (N-glycome) of Coccidioides-infected lung tissues using tandem mass spectrometry. As far as we are aware, this is the first use of mass spectrometry to compare the N-glycomes and CAZomes of different fungal genera during infection in human hosts.

INSTRUMENT(S): LTQ Orbitrap Velos, Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Lung

SUBMITTER: Natalie Mitchell

LAB HEAD: DOUGLAS LAKE

PROVIDER: PXD012459 | Pride | 2019-11-11

REPOSITORIES: Pride

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Publications

Carbo-loading in Coccidioides spp.: A quantitative analysis of CAZyme abundance and resulting glycan populations.

Mitchell Natalie M NM Grys Thomas E TE Lake Douglas F DF

Glycobiology 20200201 3

Coccidioides spp. are important pneumonia-causing pathogens of the American southwest, but little is known about their glycobiology and how their glycosylations differ from other pneumonia-causing fungi. There is mounting preliminary evidence to suggest genus or even species-specific glycosylations in the fungal kingdom due to the presence of unique carbohydrate-active enzymes (CAZymes) in fungal genomes (Deshpande et al. 2008, Glycobiology, 18(8), 626-637; Karkowska-Kuleta and Kozik 2015, Acta ...[more]

PMID: 31691790

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Dataset Information

Carbo-loading in fungal lung pathogens: a quantitative analysis of CAZyme abundance and resulting glycan populations

Publications

Carbo-loading in Coccidioides spp.: A quantitative analysis of CAZyme abundance and resulting glycan populations.

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