Proteomics

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Cysteine nitrosylation analysis in SH-SY5Y cells


ABSTRACT: Cysteine nitrosylation is emerging as important player in cellular signaling and redox homeostasis. Here we applied Cys-BOOST for quantitative analysis of nitrosylated cysteine in SNAP-treated and non-treated SH-SY5Y human neuroblastoma cells.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Ruzanna Mnatsakanyan  

LAB HEAD: René P. Zahedi

PROVIDER: PXD012485 | Pride | 2019-05-21

REPOSITORIES: Pride

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Publications

Proteome-wide detection of S-nitrosylation targets and motifs using bioorthogonal cleavable-linker-based enrichment and switch technique.

Mnatsakanyan Ruzanna R   Markoutsa Stavroula S   Walbrunn Kim K   Roos Andreas A   Verhelst Steven H L SHL   Zahedi René P RP  

Nature communications 20190516 1


Cysteine modifications emerge as important players in cellular signaling and homeostasis. Here, we present a chemical proteomics strategy for quantitative analysis of reversibly modified Cysteines using bioorthogonal cleavable-linker and switch technique (Cys-BOOST). Compared to iodoTMT for total Cysteine analysis, Cys-BOOST shows a threefold higher sensitivity and considerably higher specificity and precision. Analyzing S-nitrosylation (SNO) in S-nitrosoglutathione (GSNO)-treated and non-treate  ...[more]

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