Dataset Information

An Integrated Systems Approach Unveils New Aspects of Microoxia-mediated Regulation in Bradyrhizobium diazoefficiens

ABSTRACT: Using an integrated systems approach, the expressed proteome of B. diazoefficiens strain 110scp4 was measured under i) normal, oxic growth, and ii) microoxic growth condtions. This included, as a first step, the sequencing and de novo assembly of the genome of this widely used rhizobial model strain, which turned out to harbor several deletions and insertions compared to the B. diazoefficiens USDA 110 NCBI reference genome. With this optimal basis in hand, a shotgun proteomics approach relying on a slightly adapated FASP protocol was carried out, allowing to identify 2900 (oxia) and 2826 (microoxia) proteins, respectively, thereby largely expanding the proteome known to be expressed under microoxic conditions.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Bradyrhizobium Diazoefficiens

SUBMITTER: Christian Ahrens

LAB HEAD: Dr. Socorro Mesa

PROVIDER: PXD012491 | Pride | 2019-05-30

REPOSITORIES: Pride

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Publications

An Integrated Systems Approach Unveils New Aspects of Microoxia-Mediated Regulation in Bradyrhizobium diazoefficiens.

Fernández Noemí N Cabrera Juan J JJ Varadarajan Adithi R AR Varadarajan Adithi R AR Lutz Stefanie S Ledermann Raphael R Roschitzki Bernd B Eberl Leo L Bedmar Eulogio J EJ Fischer Hans-Martin HM Pessi Gabriella G Ahrens Christian H CH Mesa Socorro S

Frontiers in microbiology 20190507

The adaptation of rhizobia from the free-living state in soil to the endosymbiotic state comprises several physiological changes in order to cope with the extremely low oxygen availability (microoxia) within nodules. To uncover cellular functions required for bacterial adaptation to microoxia directly at the protein level, we applied a systems biology approach on the key rhizobial model and soybean endosymbiont Bradyrhizobium diazoefficiens USDA 110 (formerly B. japonicum USDA 110) ...[more]

PMID: 31134003

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Dataset Information

An Integrated Systems Approach Unveils New Aspects of Microoxia-mediated Regulation in Bradyrhizobium diazoefficiens

Publications

An Integrated Systems Approach Unveils New Aspects of Microoxia-Mediated Regulation in <i>Bradyrhizobium diazoefficiens</i>.

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