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Targeted Mass Spectrometry Analysis of Clostridium perfringens Toxins

ABSTRACT: Clostridium perfringens is an anaerobic, gram-positive, spore-forming bacterium spread throughout the environment. This bacterium is a common agent in the gastrointestinal tracts of healthy human beings and other mammals. Simultaneously, this agent is one of the most significant producers of toxins among all known bacteria. This expressive toxicity is due to the bacterium’s ability collectively to produce different protein toxins and/or enzymes with diverse modes of action. The present study uses currently developed targeted proteomic methods for the simultaneous detection of selected C. perfringens protein toxins. The method was applied in different kinds of environmental matrices and was used to analyze toxins production in a set of collection strains.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Clostridium Perfringens

SUBMITTER: Miloslava Ďuráčová

LAB HEAD: Zuzana Krocova

PROVIDER: PXD012528 | Pride | 2019-03-25

REPOSITORIES: Pride

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Targeted Mass Spectrometry Analysis of <i>Clostridium perfringens</i> Toxins.

Duracova Miloslava M Klimentova Jana J Myslivcova Fucikova Alena A Zidkova Lenka L Sheshko Valeria V Rehulkova Helena H Dresler Jiri J Krocova Zuzana Z

Toxins 20190323 3

Targeted proteomics recently proved to be a technique for the detection and absolute quantification of proteins not easily accessible to classical bottom-up approaches. Due to this, it has been considered as a high fidelity tool to detect potential warfare agents in wide spread kinds of biological and environmental matrices. <i>Clostridium perfringens</i> toxins are considered to be potential biological weapons, especially the epsilon toxin which belongs to a group of the most powerful bacterial ...[more]

PMID: 30909561

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