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Comparative O-glycoproteomics of Fusarium oxysporum

ABSTRACT: Fusarium oxysporum causes Fusarium wilt syndrome in more than 120 different plant hosts, including globally important crops such as tomato, cotton, banana, melon, etc. F. oxysporum shows high host specificity in over 150 formae speciales and have been ranked in the top 10 plant fungal pathogens. Although three PMTs encoded by the pmt1, pmt2, and pmt4 are annotated in the genome of F. oxysporum, their functions have not been reported. As O-mannosylation is not found in plants, a comprehensive understanding of PMTs in F. oxysporum becomes attractive for the development of new strategy against Fusarium wilt. In order to understand the molecular mechanism of the differential functions of three PMTs, a comparative O-glycoproteome analysis of the pmt mutants were carried out.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Fusarium Oxysporum F. Cucumerinum

SUBMITTER: Yueqiang Xu

LAB HEAD: Cheng Jin

PROVIDER: PXD012530 | Pride | 2019-10-28

REPOSITORIES: Pride

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Genetical and O-glycoproteomic analyses reveal the roles of three protein O-mannosyltransferases in phytopathogen Fusarium oxysporum f.sp. cucumerinum.

Xu Yueqiang Y Zhou Hui H Zhao Guangya G Yang Jinghua J Luo Yuanming Y Sun Shutao S Wang Zhongfu Z Li Shaojie S Jin Cheng C

Fungal genetics and biology : FG & B 20191021

Protein O-mannosyltransferases (PMTs) have been identified in fungi but not in plants and nematodes, which makes PMTs become attractive targets for developing a new strategy against phytopathogens. Three PMTs have been identified in Fusarium oxysporum, a fungal pathogen that causes vascular wilt in a broad range of economical crops. By deletion or suppression of the pmt genes, we showed that all mutants displayed retarded growth, reduced conidiation, cell wall defects, ER stress and attenuated v ...[more]

PMID: 31648060

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