Proteomics

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Phosphorus-mediated alleviation of aluminum toxicity revealed by iTRAQ technique in Citrus grandis roots


ABSTRACT: Citrus grandis seedlings were irrigated with nutrient solutions containing two levels of aluminium (Al; 0 mM and 1.2 mM AlCl3•6H2O) and two levels of phosphorus (P; 0 μM and 200 μM KH2PO4) combinations for 18 weeks, respectively. The proteome profile of Citrus root submitted to different P-Al interactions were investigated by using isobaric tags for relative and absolute quantification (iTRAQ).

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Citrus Maxima (pomelo) (citrus Grandis)

TISSUE(S): Plant Cell, Root

SUBMITTER: Lin-Tong Yang  

LAB HEAD: Lin-Tong Yang

PROVIDER: PXD012534 | Pride | 2019-11-13

REPOSITORIES: Pride

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Publications

Phosphorus-mediated alleviation of aluminum toxicity revealed by the iTRAQ technique in Citrus grandis roots.

Yang Lin-Tong LT   Zhou Yang-Fei YF   Wang Yan-Yu YY   Wu Yan-Mei YM   Qian Bing B   Wang Heng H   Chen Li-Song LS  

PloS one 20191015 10


Citrus grandis seedlings were irrigated with nutrient solutions with four Al-P combinations [two Al levels (0 mM and 1.2 mM AlCl3·6H2O) × two P levels (0 μM and 200 μM KH2PO4)] for 18 weeks. Al dramatically inhibited the growth of C. grandis seedlings, as revealed by a decreased dry weight of roots and shoots. Elevating P level could ameliorate the Al-induced growth inhibition and organic acid (malate and citrate) secretion in C. grandis. Using a comparative proteomic approach revealed by the is  ...[more]

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