Dataset Information

Assessment of protein extraction and digestion efficiency of well-established shotgun protocols for heart proteomics

ABSTRACT: In this study, we evaluated three well-established sample preparation methods for shotgun proteomics, named: i) UREA; ii) sodium dodecyl sulfate (SDS); and iii) filter-aided sample preparation (FASP) protocols. We focused our analysis on protein extraction and digestion from heart left ventricle (LV) tissue.The three methods were compared by relative quantification using dimethyl labeling.

INSTRUMENT(S):

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Heart

SUBMITTER: Thiago Verano-Braga