Dataset Information

Pacific halibut white muscle DDA proteomics

ABSTRACT: White muscle samples from juvenile Pacific halibut were analyzed in discovery-driven proteomics to investigate the effects of temperature-induced growth manipulations and to identify protein markers of somatic growth. Juvenile Pacific halibut were either acclimated to 2C (low temperature) and 9C (control temperature) to elicit growth suppression and also initially acclimated to 2C and subsequently transferred to 9C to elicit growth compensation. This project was led by Josep Planas at the International Pacific Halibut Commission.

INSTRUMENT(S):

ORGANISM(S): Hippoglossus Stenolepis

TISSUE(S): Muscle

SUBMITTER: Emma Timmins-Schiffman

LAB HEAD: Brook Nunn

PROVIDER: PXD012634 | Pride | 2019-10-11

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20181126_UWPRQE_halibut1.pep.xml	Pepxml
	20181126_UWPRQE_halibut1.raw	Raw
	20181126_UWPRQE_halibut10.pep.xml	Pepxml
	20181126_UWPRQE_halibut10.raw	Raw
	20181126_UWPRQE_halibut11.pep.xml	Pepxml

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Project description:During low temperature exposure, temperate plant species increase their freezing tolerance in a process termed cold acclimation. During deacclimation in response to warm temperatures cold acclimated plants lose freezing tolerance and resume growth and development. While considerable effort has been directed toward understanding the molecular and metabolic basis of cold acclimation, much less information is available about the regulation of deacclimation. Here, we report metabolic (GC-MS) and transcriptional (microarrays, qRT-PCR) responses underlying deacclimation during the first 24 h after a shift of cold acclimated Arabidopsis thaliana (Columbia-0) plants to warm temperature. The data revealed a faster response of the transcriptome than of the metabolome and provided evidence for tightly regulated temporal responses at both levels. Metabolically deacclimation is associated with decreasing contents of sugars, amino acids and glycolytic and TCA cycle intermediates, indicating an increased need for carbon sources and respiratory energy production associated with growth resumption under warm temperature conditions. Deacclimation also involves extensive down-regulation of protein synthesis and changes in the metabolism of lipids and cell wall components. Altered hormonal regulation appears particularly important during deacclimation, with changes in the expression of genes related to auxin, gibberellin, brassinosteroid, jasmonate and ethylene metabolisms. Several transcription factor families controlling fundamental aspects of plant development are significantly regulated during deacclimation, emphasizing that loss of freezing tolerance and growth resumption are interrelated processes that are transcriptionally highly interrelated. Expression patterns of some clock oscillator components during deacclimation resembled those under warm conditions, indicating at least partial re-activation of the circadian clock. This study provide the first comprehensive analysis of the regulation of deacclimation in cold acclimated plants. The data indicate cascades of rapidly regulated genes and metabolites that underly the developmental switch resulting in reduced freezing tolerance and the resumption of growth. They constitute a reference dataset of genes, metabolites and pathways that are crucial during the first rapid phase of deacclimation and will be useful for the further analysis of this important but under-researched plant process. We used whole genome microarrays to monitor changes in gene expression in the Arabidopsis thaliana accession Columbia-0 during the first 24 h after a shift of cold acclimated plants to warm temperature.

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Pacific halibut white muscle DDA proteomics

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