Dataset Information

An updated perspective on deconvoluting chimeric MS/MS spectra by LC and precursor isolation and their subsequent assignment by the CharmeRT mPSM search strategy

ABSTRACT: Considering the recent advances in LC and mPSM methodologies, we aimed to provide a systematic comparison and in-depth analysis of the effects that standard and specialized LC setups, as well as different isolation windows, have on the occurrence and assignment of chimeric spectra derived from a complex peptide mixture. To this end, we provide a comprehensive analysis of how these factors influence the chromatographic resolution, isolation interference, and the subsequent identification of MS/MS spectra by a traditional or mPSM algorithm.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell

SUBMITTER: Paul Abraham

LAB HEAD: Paul Abraham

PROVIDER: PXD012635 | Pride | 2019-05-20

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	11082018_easy_nlc_HELA_standard_2ug_500min_iw08_c18_HPLC_ac50cm_AP.msf	Msf
	11082018_easy_nlc_HELA_standard_2ug_500min_iw30_c18_HPLC_ac50cm_AP.msf	Msf
	20181019_HeLa_Std_2ug_2D_RP210_IW16_ENL_QE1_0_AP.msf	Msf
	20181019_HeLa_Std_2ug_Run2_2D_RP210_ENL_QE1_02.raw	Raw
	20181019_HeLa_Std_2ug_Run2_2D_RP210_ENL_QE1_02_CharmeRT_with_Model.msf	Msf

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Dataset Information

An updated perspective on deconvoluting chimeric MS/MS spectra by LC and precursor isolation and their subsequent assignment by the CharmeRT mPSM search strategy

Dataset's files

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