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LC-MS/MS proteomics analysis of Panc-1 EVs isolated using SEC

ABSTRACT: LC-MS/MS proteomics analysis of Panc-1 EVs isolated using SEC

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell, Epithelial Cell, Cell Culture

DISEASE(S): Pancreatic Ductal Adenocarcinoma

SUBMITTER: Krystine Mansfield

LAB HEAD: Patrick Pirrotte

PROVIDER: PXD012660 | Pride | 2019-10-24

REPOSITORIES: Pride

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Single molecule characterization of individual extracellular vesicles from pancreatic cancer.

Lennon Kathleen M KM Wakefield Devin L DL Maddox Adam L AL Brehove Matthew S MS Willner Ari N AN Garcia-Mansfield Krystine K Meechoovet Bessie B Reiman Rebecca R Hutchins Elizabeth E Miller Marcia M MM Goel Ajay A Pirrotte Patrick P Van Keuren-Jensen Kendall K Jovanovic-Talisman Tijana T

Journal of extracellular vesicles 20191104 1

Biofluid-accessible extracellular vesicles (EVs) may represent a new means to improve the sensitivity and specificity of detecting disease. However, current methods to isolate EVs encounter challenges when they are used to select specific populations. Moreover, it has been difficult to comprehensively characterize heterogeneous EV populations at the single vesicle level. Here, we robustly assessed heterogeneous EV populations from cultured cell lines via nanoparticle tracking analysis, proteomic ...[more]

PMID: 31741725

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