Name: Oxidative Stress Biomarker of Type 2 Diabetes Mellitus
Keywords: Human plasma

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Oxidative Stress Biomarker of Type 2 Diabetes Mellitus

ABSTRACT: In this project, using discovery-based mass spectrometry approach and adopting data-dependent LC-MS/MS, we detected and identified modified peptides in complex clinical plasma samples and developed first-level biomarker verification strategy. Further, using liquid chromatography-multiple reaction monitoring mass spectrometry (LC-MRM-MS) based targeted approach which virtually quantifies irreversible oxidation at any targeted cysteine, we quantified cysteine tri-oxidation in HSA in diabetes patients. Site-specific tri-oxidized HSA could be a potential biomarker of diabetes and provide directive of disease mechanism.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Cell, Blood Plasma

DISEASE(S): Type 2 Diabetes Mellitus

SUBMITTER: Sunil Adav

LAB HEAD: Prof Sze S K

PROVIDER: PXD012667 | Pride | 2020-05-26

REPOSITORIES: Pride

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Serum albumin cysteine trioxidation is a potential oxidative stress biomarker of type 2 diabetes mellitus.

Paramasivan Selvam S Adav Sunil S SS Ngan SoFong Cam SC Dalan Rinkoo R Leow Melvin Khee-Shing MK Ho Hee Hwa HH Sze Siu Kwan SK

Scientific reports 20200415 1

Metabolic disorders in T2DM generate multiple sources of free radicals and oxidative stress that accelerate nonenzymatic degenerative protein modifications (DPMs) such as protein oxidation, disrupt redox signaling and physiological function, and remain a major risk factor for clinical diabetic vascular complications. In order to identify potential oxidative biomarkers in the blood plasma of patients with T2DM, we used LC-MS/MS-based proteomics to profile plasma samples from patients with T2DM an ...[more]

PMID: 32296090

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