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Global effects of pathway-specific regulator CpkO and CpkN deletion in Streptomyces coelicolor A3(2)

ABSTRACT: Relative quantification of protein abundances of S. coelicolor A3(2) parent strain M145 and deletion mutants ∆cpkO and ∆cpkN. This study shows novel insights into the mechanism of coelimycin synthesis regulation and the network of interactions between cpk cluster regulatory proteins and the biosythetic genes of other secondary metabolite clusters.

INSTRUMENT(S):

ORGANISM(S): Streptomyces Coelicolor A3(2)

TISSUE(S): Prokaryotic Cell

SUBMITTER: Aaron Millan Oropeza

LAB HEAD: Celine Henry

PROVIDER: PXD012672 | Pride | 2021-03-29

REPOSITORIES: Pride

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			Action	DRS
	20181023_04_P193_A.mzXML	Mzxml
	20181023_04_P193_A.xml	Xml
	20181023_06_P196_A.mzXML	Mzxml
	20181023_06_P196_A.xml	Xml
	20181023_08_P193_B.mzXML	Mzxml

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Relative quantification from chemostats cultures of Saccharomyces cerevisiae CEN.PK113-7D, Kluyveromyces marxianus CBS 6556 and Yarrowia lipolytica W29

Project description:Relative quantification of protein abundances of three yeast strains (Saccharomyces cerevisiae CEN.PK113-7D, Kluyveromyces marxianus CBS6556 and Yarrowia lipolytica W29) cultivate in chemostats under different conditions. The conditions for Saccharomyces cerevisiae CEN.PK113-7D are: - Standard condition – 30°C, pH 5.5 - High temperature - 36°C, pH 5.5 - Low pH - 30°C, pH 3.5 - Osmotic stress – 30°C, pH 5.5, 1M KCl The conditions for Kluyveromyces marxianus CBS6556 are: - Standard condition – 30°C, pH 5.5 - High temperature - 40°C, pH 5.5 - Low pH - 30°C, pH 3.5 - Osmotic stress – 30°C, pH 5.5, 0.6 M KCl The conditions for Yarrowia lipolytica W29 are: - Standard condition - 28°C, pH 5.5 - High temperature - 32°C, pH 5.5 - Low pH - 28°C, pH 3.5 This study is part of the OMICS data generation of CHASSY project (European Union’s Horizon 2020 grant agreement No 720824).

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RNA-Sequenzing of Streptomyces coelicolor A3(2) (M1152) in timeseries

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