Semi-quantitative proteomics enables mapping of global neutrophil dynamics following influenza virus infection

ABSTRACT: Lung neutrophils are causally associated with IAV-induced disease severity. Less is known about the repertoire of lethal IAV-associated neutrophil proteins or about how global changes in different neutrophil compartments are coordinated following lethal IAV infection. Here, we use semi-quantitative proteomics to characterize dynamic alterations in BM, blood and lung neutrophils at homeostasis or following a lethal IAV infection, with a secondary aim of identifying lung neutrophil-derived proteins which are selectively induced following IAV infection. Our findings identify bone marrow neutrophil maturation as the key site of anti-viral activity induction, with further upregulation or release of both anti-viral and antimicrobial effectors occurring following lung tissue infiltration.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Bronchoalveolar Lavage, Neutrophil, Bone Marrow, Blood

DISEASE(S): Influenza

SUBMITTER: Chuanxin Liu  

LAB HEAD: Weisan Chen

PROVIDER: PXD012681 | Pride | 2019-05-29

REPOSITORIES: Pride