Proteomics

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Pax3 interactome in mouse ES cell-derived mesodermal precursors

ABSTRACT: The skeletal myogenic lineage in developing embryos is specified within the somites and requires members of the Paired box (Pax) family of transcription factors. In particular, Pax3 is the first member of this family expressed in the central region of the dermomyotome and it is necessary for specification, migration and survival of the myogenic progenitors that will form the muscles of the limbs, diaphragm and tongue. To investigate Pax3 molecular functions in the developing mesoderm, we generated doxycycline-inducible mouse embryonic stem (ES) cells expressing untagged and HaFlag-tagged-Pax3. These cell lines were used to unbiasedly identify its interacting partners by tandem affinity purification followed by mass spectrometry.

OTHER RELATED OMICS DATASETS IN: GSE126362

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Trunk Somite, Mesoderm, Myotome, Dermomyotome, Embryonic Stem Cell, Paraxial Mesoderm

SUBMITTER: Alessandro Magli  

LAB HEAD: Rita CR Perlingeiro

PROVIDER: PXD012693 | Pride | 2019-04-18

REPOSITORIES: Pride

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Publications

Pax3 cooperates with Ldb1 to direct local chromosome architecture during myogenic lineage specification.

Magli Alessandro A   Baik June J   Pota Pruthvi P   Cordero Carolina Ortiz CO   Kwak Il-Youp IY   Garry Daniel J DJ   Love Paul E PE   Dynlacht Brian D BD   Perlingeiro Rita C R RCR  

Nature communications 20190524 1

Chromatin looping allows enhancer-bound regulatory factors to influence transcription. Large domains, referred to as topologically associated domains, participate in genome organization. However, the mechanisms underlining interactions within these domains, which control gene expression, are not fully understood. Here we report that activation of embryonic myogenesis is associated with establishment of long-range chromatin interactions centered on Pax3-bound loci. Using mass spectrometry and gen  ...[more]

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