Pax3 interactome in mouse ES cell-derived mesodermal precursors

ABSTRACT: The skeletal myogenic lineage in developing embryos is specified within the somites and requires members of the Paired box (Pax) family of transcription factors. In particular, Pax3 is the first member of this family expressed in the central region of the dermomyotome and it is necessary for specification, migration and survival of the myogenic progenitors that will form the muscles of the limbs, diaphragm and tongue. To investigate Pax3 molecular functions in the developing mesoderm, we generated doxycycline-inducible mouse embryonic stem (ES) cells expressing untagged and HaFlag-tagged-Pax3. These cell lines were used to unbiasedly identify its interacting partners by tandem affinity purification followed by mass spectrometry.

OTHER RELATED OMICS DATASETS IN: GSE126362

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Trunk Somite, Mesoderm, Myotome, Dermomyotome, Embryonic Stem Cell, Paraxial Mesoderm

SUBMITTER: Alessandro Magli  

LAB HEAD: Rita CR Perlingeiro

PROVIDER: PXD012693 | Pride | 2019-04-18

REPOSITORIES: Pride