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Synovial fluid proteome changes in ACL injury-induced posttraumatic osteoarthritis: proteomics analysis of porcine knee synovial fluid

ABSTRACT: In the current work, we used an isotopically labeled high resolution LC MS/MS-based proteomics approach to analyze the protein profile of synovial fluid at 6 and 12 months after ACL transection in untreated and repaired porcine knees. Our primary aim was to determine how the synovial fluid proteome differs between the two groups in an effort to identify candidate proteins that may be associated with the development of posttraumatic OA. We hypothesized that the development of macroscopic cartilage damage following surgical ACL transection would be accompanied by differential changes in synovial fluid proteome in untreated knees compared to repaired joints.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Sus Scrofa Domesticus (domestic Pig)

SUBMITTER: Ata Kiapour

LAB HEAD: Ata Kiapour

PROVIDER: PXD012720 | Pride | 2019-02-16

REPOSITORIES: Pride

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Synovial fluid proteome changes in ACL injury-induced posttraumatic osteoarthritis: Proteomics analysis of porcine knee synovial fluid.

Kiapour Ata M AM Sieker Jakob T JT Proffen Benedikt L BL Lam TuKiet T TT Fleming Braden C BC Murray Martha M MM

PloS one 20190301 3

Surgical transection of the anterior cruciate ligament (ACL) in the porcine model leads to posttraumatic osteoarthritis if left untreated. However, a recently developed surgical treatment, bridge-enhanced ACL repair, prevents further cartilage damage. Since the synovial fluid bathes all the intrinsic structures of knee, we reasoned that a comparative analysis of synovial fluid protein contents could help to better understand the observed chondroprotective effects of the bridge-enhanced ACL repai ...[more]

PMID: 30822327

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