Proteomics

Dataset Information

0

USP9X phosphopeptide mapping - We previously identified the deubiquitylase USP9X as a G2/M-specific CDC14B interactor (PXD012732). The functional connection of these proteins has been further investigated by a quantitative comparison of USP9X phosphorylation in WT vs CDC14B-overexpressing HEK 293Tcells (ATCC® CRL-3216™). The approach identified USP9X serine 2563 as a specific CDC14B target. Phosphorylation at USP9XS2563 has been confirmed by in-house phosphospecific antibodies.

ABSTRACT: We previously identified the deubiquitylase USP9X as a G2/M-specific CDC14B interactor (PXD012732). The functional connection of these proteins has been further investigated by a quantitative comparison of USP9X phosphorylation in WT vs CDC14B-overexpressing HEK 293Tcells (ATCC® CRL-3216™). The approach identified USP9X serine 2563 as a specific CDC14B target. Phosphorylation at USP9XS2563 has been confirmed by in-house phosphospecific antibodies.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Johannes Gloeckner  

LAB HEAD: Christian Johannes Gloeckner

PROVIDER: PXD012733 | Pride | 2020-03-25

REPOSITORIES: Pride

Json Xml
altmetric image

Publications

Regulation of mitosis secures cellular integrity and its failure critically contributes to the development, maintenance, and treatment resistance of cancer. In yeast, the dual phosphatase Cdc14 controls mitotic progression by antagonizing Cdk1-mediated protein phosphorylation. By contrast, specific mitotic functions of the mammalian Cdc14 orthologue CDC14B have remained largely elusive. Here, we find that CDC14B antagonizes CDK1-mediated activating mitotic phosphorylation of the deubiquitinase U  ...[more]

Similar Datasets

Proteomics Identification of mitotic substrates of phospho-regulated USP9X
2020-03-25 | PXD012734 | Pride
Proteomics,Multiomics CDC14B interactome - The proteomic approach aimed at the identification of interaction partners for the human dual phosphatase and tumor suppressor CDC14B by Co-inmunoprecipitation (Co-IP) experiments. In this approach, CDC14B interactomes from unsynchronized and G2/M enriched cells were compared semi-quantitatively based on spectral counting. This approach yielded the deubiquitylase USP9X as a G2/M-specific CDC14B interactor which has been confirmed by Western-blot (WB).
2020-03-25 | PXD012732 | Pride
Transcriptomics Loss of Usp9x increases Wnt and Notch signaling in neural progenitors
2017-08-18 | E-MTAB-4150 | biostudies-arrayexpress
Unknown The deubiquitinase Usp9x regulates PRC2-mediated chromatin reprogramming during mouse development
2021-01-25 | GSE146800 | GEO
Proteomics Identification of a USP9X Substrate NFX1-123 by SILAC-based Quantitative Proteomics
2019-05-13 | PXD012871 | Pride
Unknown USP9X stabilizes EWS-FLI1 to promote cancer cell growth in Ewing’s sarcoma
2022-01-01 | GSE185429 | GEO
Unknown Nitric oxide prevents aortic valve calcification by S-nitrosylation of USP9X to activate NOTCH signaling
2020-12-22 | GSE161123 | GEO
Unknown USP9X-mediated KDM4C deubiquitination promotes lung cancer radioresistance by epigenetically inducing TGFβ2 transcription
2021-07-14 | GSE136404 | GEO
Proteomics USP9X label-free quantitative proteomics
2021-09-07 | PXD028223 | iProX
Proteomics Deubiquitinase USP9X loss sensitizes renal cancer cells to mTOR inhibition
2023-06-14 | PXD040496 | Pride