Zebrafish, LC-MS/MS - Profiling of Histidine Phosphoproteome in Danio rerio by TiO2 Enrichment

ABSTRACT: Histidine phosphorylation is a reversible post-translational modification that is known to regulate signal transduction in prokaryotes. In an effort to help elucidate the heretofore hidden vertebrate phosphoproteome, this report presents a global phosphorylation analysis of Danio rerio (zebrafish) larvae. Phosphopeptide enrichment was performed using a TiO2 affinity technique. A total of 68 unique phosphohistidine sites were detected on 63 proteins among 1076 unique phosphosites on 708 proteins. This report provides the first phosphohistidine dataset obtained from zebrafish.

INSTRUMENT(S):

ORGANISM(S): Danio Rerio (zebrafish) (brachydanio Rerio)

TISSUE(S): Embryo

SUBMITTER: Yan Gao  

LAB HEAD: Sangkyu Lee

PROVIDER: PXD012735 | Pride | 2019-03-21

REPOSITORIES: Pride