Dataset Information

Barnacle cyprid cement gland LC-MS/MS

ABSTRACT: Proteins were extracted from the cement glands of the cypird barnacle Megabalanus volcano. And thenproteins were subjected to in-solution digestion and analyzed by LTQ-Orbitrap Elite coupled to an Easy-nLC.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Megabalanus Volcano

TISSUE(S): Cement Gland

SUBMITTER: Guoyong Yan

LAB HEAD: Lisheng He

PROVIDER: PXD012779 | Pride | 2021-09-08

REPOSITORIES: Pride

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Publications

Insights into the Synthesis, Secretion and Curing of Barnacle Cyprid Adhesive via Transcriptomic and Proteomic Analyses of the Cement Gland.

Yan Guoyong G Sun Jin J Wang Zishuai Z Qian Pei-Yuan PY He Lisheng L

Marine drugs 20200331 4

Barnacles represent one of the model organisms used for antifouling research, however, knowledge regarding the molecular mechanisms underlying barnacle cyprid cementation is relatively scarce. Here, RNA-seq was used to obtain the transcriptomes of the cement glands where adhesive is generated and the remaining carcasses of <i>Megabalanus</i> <i>volcano</i> cyprids. Comparative transcriptomic analysis identified 9060 differentially expressed genes, with 4383 upregulated in the cement glands. Four ...[more]

PMID: 32244485

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Project description:ABSTRACT: Acorn barnacles are efficient colonizers on a wide variety of marine surfaces. As they proliferate on critical infrastructure, their settlement and growth have deleterious effects on performance. To address acorn barnacle biofouling, research has focused on the settlement and adhesion processes with the goal of informing the development of novel coatings. This effort has resulted in the discovery and characterization of several proteins found at the adhesive substrate interface, i.e. cement proteins, and a deepened understanding of the function and composition of the biomaterials within this region. While the adhesive properties at the interface are affected by the interaction between the proteins, substrate, and mechanics of the calcified base plate, little attention has been given to the interaction between the proteins and the cuticular material present at the substrate interface. Here, the proteome of the organic matrix isolated from the base plate of the acorn barnacle Amphibalanus amphitrite is compared with the chitinous and proteinaceous matrix embedded within A. amphitrite parietal plates. The objective was to gain an understanding of how the basal organic matrix may be specialized for adhesion via an in-depth comparative proteome analysis. In general, the majority of proteins identified in the parietal matrix were also found in the basal organic matrix, including nearly all those grouped in classes of cement proteins, enzymes and pheromones. However, the parietal organic matrix was enriched with cuticle-associated proteins, of which ~30% of those identified were unique to the parietal region. In contrast, ~30-40% of the protease inhibitors, enzymes, and pheromones identified in the basal organic matrix were unique to this region. Not unexpectedly, nearly 50% of the cement proteins identified in the basal region were significantly distinct from those found in the parietal region. The wider variety of identified proteins in the basal organic matrix indicates a greater diversity of biological function in the vicinity of the substrate interface where several processes related to adhesion, cuticle formation, and expansion of the base synchronize to play a key role in organism survival.

Dataset Information

Barnacle cyprid cement gland LC-MS/MS

Publications

Insights into the Synthesis, Secretion and Curing of Barnacle Cyprid Adhesive via Transcriptomic and Proteomic Analyses of the Cement Gland.

Similar Datasets

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