Ontology highlight
ABSTRACT:
INSTRUMENT(S): Q Exactive
ORGANISM(S): Sulfolobus Islandicus Lal14/1
TISSUE(S): Cell Suspension Culture
SUBMITTER: JJ Park
LAB HEAD: Edward H. Egelman
PROVIDER: PXD012799 | Pride | 2020-05-26
REPOSITORIES: Pride
Wang Fengbin F Cvirkaite-Krupovic Virginija V Kreutzberger Mark A B MAB Su Zhangli Z de Oliveira Guilherme A P GAP Osinski Tomasz T Sherman Nicholas N DiMaio Frank F Wall Joseph S JS Prangishvili David D Krupovic Mart M Egelman Edward H EH
Nature microbiology 20190520 8
Pili on the surface of Sulfolobus islandicus are used for many functions, and serve as receptors for certain archaeal viruses. The cells grow optimally at pH 3 and ~80 °C, exposing these extracellular appendages to a very harsh environment. The pili, when removed from cells, resist digestion by trypsin or pepsin, and survive boiling in sodium dodecyl sulfate or 5 M guanidine hydrochloride. We used electron cryo-microscopy to determine the structure of these filaments at 4.1 Å resolution. An atom ...[more]