Ontology highlight
ABSTRACT:
INSTRUMENT(S): Orbitrap Fusion ETD
ORGANISM(S): Homo Sapiens (human)
SUBMITTER: Cristina Furlan
LAB HEAD: Michiel Vermeulen
PROVIDER: PXD012800 | Pride | 2019-04-02
REPOSITORIES: Pride
Furlan Cristina C Dirks René A M RAM Thomas Peter C PC Jones Robert C RC Wang Jing J Lynch Mark M Marks Hendrik H Vermeulen Michiel M
Nature communications 20190404 1
Essentially all cellular processes are orchestrated by protein-protein interactions (PPIs). In recent years, affinity purification coupled to mass spectrometry (AP-MS) has been the preferred method to identify cellular PPIs. Here we present a microfluidic-based AP-MS workflow, called on-chip AP-MS, to identify PPIs using minute amounts of input material. By using this automated platform we purify the human Cohesin, CCC and Mediator complexes from as little as 4 micrograms of input lysate, repres ...[more]