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TMT and Label-Free Quantification of Yeast Mitochondrial and Cellular Proteome During Different Stages of Growth

ABSTRACT: Tandem mass tag (TMT)-based relative quantification was used in combination with the intensity-based absolute quantity estimation (IBAQ) to study of both yeast cells and isolated yeast mitochondria during fermentative growth, diauxic shift, respiratory growth.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: Egor Vorontsov

LAB HEAD: Jens Nielsen

PROVIDER: PXD012802 | Pride | 2020-03-06

REPOSITORIES: Pride

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Absolute yeast mitochondrial proteome quantification reveals trade-off between biosynthesis and energy generation during diauxic shift.

Di Bartolomeo Francesca F Malina Carl C Campbell Kate K Mormino Maurizio M Fuchs Johannes J Vorontsov Egor E Gustafsson Claes M CM Nielsen Jens J

Proceedings of the National Academy of Sciences of the United States of America 20200317 13

<i>Saccharomyces cerevisiae</i> constitutes a popular eukaryal model for research on mitochondrial physiology. Being Crabtree-positive, this yeast has evolved the ability to ferment glucose to ethanol and respire ethanol once glucose is consumed. Its transition phase from fermentative to respiratory metabolism, known as the diauxic shift, is reflected by dramatic rearrangements of mitochondrial function and structure. To date, the metabolic adaptations that occur during the diauxic shift have no ...[more]

PMID: 32184324

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