Proteomics

Dataset Information

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Label-Free Quantification of Yeast Cellular Proteome


ABSTRACT: Precursor ion intensity quantification and intensity-based absolute quantity estimation (IBAQ) was used to on the mixture of yeast cell lysates in order to estimate the absolute concentrations of the proteins.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: Egor Vorontsov  

LAB HEAD: Jens Nielsen

PROVIDER: PXD012803 | Pride | 2020-03-06

REPOSITORIES: Pride

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Publications

Absolute yeast mitochondrial proteome quantification reveals trade-off between biosynthesis and energy generation during diauxic shift.

Di Bartolomeo Francesca F   Malina Carl C   Campbell Kate K   Mormino Maurizio M   Fuchs Johannes J   Vorontsov Egor E   Gustafsson Claes M CM   Nielsen Jens J  

Proceedings of the National Academy of Sciences of the United States of America 20200317 13


<i>Saccharomyces cerevisiae</i> constitutes a popular eukaryal model for research on mitochondrial physiology. Being Crabtree-positive, this yeast has evolved the ability to ferment glucose to ethanol and respire ethanol once glucose is consumed. Its transition phase from fermentative to respiratory metabolism, known as the diauxic shift, is reflected by dramatic rearrangements of mitochondrial function and structure. To date, the metabolic adaptations that occur during the diauxic shift have no  ...[more]

Publication: 1/3

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