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Optimization of HUNTER N-termini profiling using Arabidopsis thaliana Col-8 wt leaf proteome extracts

ABSTRACT: Optimization hydrophobic tagging reagent (hexadecanal or undecanal), reaction time (30 min to 16h) and evaluation of the HUNTER protocol performance with different amounts (25 ug to 400 ug) of purified Arabidopsis thaliana Col-8 wt leaf proteome as starting material.

INSTRUMENT(S): maXis

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Plant Cell, Leaf

SUBMITTER: Fatih Demir

LAB HEAD: Pitter Florian Huesgen

PROVIDER: PXD012821 | Pride | 2019-09-03

REPOSITORIES: Pride

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Sensitive Determination of Proteolytic Proteoforms in Limited Microscale Proteome Samples.

Weng Samuel S H SSH Demir Fatih F Ergin Enes K EK Dirnberger Sabrina S Uzozie Anuli A Tuscher Domenic D Nierves Lorenz L Tsui Janice J Huesgen Pitter F PF Lange Philipp F PF

Molecular & cellular proteomics : MCP 20190830 11

Protein N termini unambiguously identify truncated, alternatively translated or modified proteoforms with distinct functions and reveal perturbations in disease. Selective enrichment of N-terminal peptides is necessary to achieve proteome-wide coverage for unbiased identification of site-specific regulatory proteolytic processing and protease substrates. However, many proteolytic processes are strictly confined in time and space and therefore can only be analyzed in minute samples that provide i ...[more]

PMID: 31471496

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