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Transfer of antigen target into CAR T cells via trogocytosis

ABSTRACT: To characterize transfer of molecules from target cells into CAR T cells via trogocytosis we cultured NALM-6 leukemia cell line expressing a CD19-mCherry fusion protein with CAR T cells. NALM6-CD19-mCherry were loaded with heavy amino acid and cocultured with CAR T cells for 1 hour. CAR T cells were next sorted into two fractions, mCherry-positive (TrogPos), and -negative (TrogNeg). Proteomics analysis revealed the presence of targeted antigen (CD19) in the TrogPos only.

OTHER RELATED OMICS DATASETS IN: GSE126753

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): T Cell

DISEASE(S): Acute Leukemia

SUBMITTER: Zhuoning Li

LAB HEAD: Michel Sadelain

PROVIDER: PXD012833 | Pride | 2019-03-29

REPOSITORIES: Pride

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CAR T cell trogocytosis and cooperative killing regulate tumour antigen escape.

Hamieh Mohamad M Dobrin Anton A Cabriolu Annalisa A van der Stegen Sjoukje J C SJC Giavridis Theodoros T Mansilla-Soto Jorge J Eyquem Justin J Zhao Zeguo Z Whitlock Benjamin M BM Miele Matthew M MM Li Zhuoning Z Cunanan Kristen M KM Huse Morgan M Hendrickson Ronald C RC Wang Xiuyan X Rivière Isabelle I Sadelain Michel M

Nature 20190327 7750

Chimeric antigen receptors (CARs) are synthetic antigen receptors that reprogram T cell specificity, function and persistence1. Patient-derived CAR T cells have demonstrated remarkable efficacy against a range of B-cell malignancies1-3, and the results of early clinical trials suggest activity in multiple myeloma4. Despite high complete response rates, relapses occur in a large fraction of patients; some of these are antigen-negative and others are antigen-low<su ...[more]

PMID: 30918399

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CAR T cells inhibit B cell activating phosphorylation in their targets

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