Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Optimization of HUNTER N-termini profiling using rat brain proteome extracts

ABSTRACT: Optimization hydrophobic tagging reaction time (30 min to 2h) and evaluation of the HUNTER protocol performance with different amounts (25 ug to 400 ug) of purified rat brain proteome as starting material.

INSTRUMENT(S): maXis

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Brain

SUBMITTER: Fatih Demir

LAB HEAD: Pitter Florian Huesgen

PROVIDER: PXD012844 | Pride | 2019-09-03

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Publications

Sensitive Determination of Proteolytic Proteoforms in Limited Microscale Proteome Samples.

Weng Samuel S H SSH Demir Fatih F Ergin Enes K EK Dirnberger Sabrina S Uzozie Anuli A Tuscher Domenic D Nierves Lorenz L Tsui Janice J Huesgen Pitter F PF Lange Philipp F PF

Molecular & cellular proteomics : MCP 20190830 11

Protein N termini unambiguously identify truncated, alternatively translated or modified proteoforms with distinct functions and reveal perturbations in disease. Selective enrichment of N-terminal peptides is necessary to achieve proteome-wide coverage for unbiased identification of site-specific regulatory proteolytic processing and protease substrates. However, many proteolytic processes are strictly confined in time and space and therefore can only be analyzed in minute samples that provide i ...[more]

PMID: 31471496

Similar Datasets

Optimization of HUNTER N-termini profiling using Arabidopsis thaliana Col-8 wt leaf proteome extracts

Project description:Optimization hydrophobic tagging reagent (hexadecanal or undecanal), reaction time (30 min to 16h) and evaluation of the HUNTER protocol performance with different amounts (25 ug to 400 ug) of purified Arabidopsis thaliana Col-8 wt leaf proteome as starting material.

2019-09-03 | PXD012821 | Pride

HUNTER N-termini profiling of Arabidopsis thaliana leaves using different proteases

Project description:Comparison of protein N-termini in Arabidopsis thaliana leaves by the HUNTER method; the corresponding digestion was performed with legumain, GluC and trypsin.

2020-01-20 | PXD014697 | Pride

Arabidopsis FTSH12 mutant terminomics

Project description:DEgradomic analysis of protein termini in Arabidopsis thaliana mutants expressing altered abundance of the chloroplast protease FTSH12.

2020-11-09 | PXD022423 | Pride

HUNTER N-termini profiling of Arabidopsis thaliana wt and vpe0 mutants

Project description:Comparison of protein termini in Arabidopsis thaliana vpe0 quadruple mutant and wildtype seedlings shortly after germination to identify differential processed proteins.

2019-09-03 | PXD012804 | Pride

Complete Genomes of a Hunter-Gatherer and a Bantu from Southern Africa

Project description:The genetic structure of the indigenous hunter-gatherer peoples of Southern Africa, the oldest known lineage of modern man, holds an important key to understanding humanity's early history. Previously sequenced human genomes have been limited to recently diverged populations. Here we present the first complete genome sequences of an indigenous hunter-gatherer from the Kalahari Desert and of a Bantu from Southern Africa, as well as protein-coding regions from an additional three hunter-gatherers from disparate regions of the Kalahari. We characterize the extent of whole-genome and exome diversity among the five men, reporting 1.3 million novel DNA differences genome-wide, and 13,146 novel amino-acid variants. These data allow genetic relationships among Southern African foragers and neighboring agriculturalists to be traced more accurately than was previously possible. Adding the described variants to current databases will facilitate inclusion of Southern Africans in medical research efforts.

2010-02-18 | GSE19048 | GEO

Natural selection has contributed to functional immune response differences between human hunter-gatherers and agriculturalists

Project description:The shift from a hunter-gatherer (HG) to an agricultural (AG) mode of subsistence is believed to have been associated with profound changes in the burden and diversity of pathogens across human populations. Yet, the extent to which the advent of agriculture impacted the evolution of the human immune system remains unknown. Here we present a comparative study of variation in the transcriptional responses of peripheral blood mononuclear cells (PBMCs) to bacterial and viral stimuli between the Batwa, a rainforest hunter-gatherer, and the Bakiga, an agriculturalist population from Central Africa. We observed increased divergence between hunter-gatherers and farmers in the transcriptional response to viruses compared to that for bacterial stimuli. We demonstrate that a significant fraction of these transcriptional differences are under genetic control, and we show that positive natural selection has helped to shape population differences in immune regulation. Unexpectedly, we found stronger signatures of recent natural selection in the rainforest hunter-gatherers, which argues against the popularized notion that shifts in pathogen exposure due to the advent of agriculture imposed radically heightened selective pressures in agriculturalist populations.

2018-09-27 | GSE120502 | GEO

Complete Genomes of a Hunter-Gatherer and a Bantu from Southern Africa

2010-02-17 | E-GEOD-19048 | biostudies-arrayexpress

Genome wide evidence of Austronesian-Bantu admixture and cultural reversion in a hunter-gatherer group of Madagascar

Project description:Linguistic and cultural evidence suggests that Madagascar was the final point of two major dispersals of Austronesian and Bantu speaking populations. Today, Mikea are described as the last known Malagasy population reported to be still practising a hunter-gatherer life-style. It is unclear, however, whether Mikea descend from a remnant population that existed before the arrival of Austronesian and Bantu agriculturalists or whether it is only their life-style that separates them from the other contemporary populations of South Madagascar. In order to address these questions we have performed a genome wide analysis of >700,000 SNP markers on 21 Mikea, 24 Vezo and 24 Temoro individuals together with 50 individuals from Bajo and Lebbo populations from Indonesia. Our analyses of these data in the context of data available from other Southeast Asian and African populations reveal that all three Malagasy populations are derived from the same admixture event involving Austronesian and Bantu sources. In contrast to the fact that most of the vocabulary of the Malagasy speakers is derived from the Barito group of the Austronesian language family we observe that only one third of their genetic ancestry is related to the populations of Java-Kalimantan-Sulawesi area. Because no additional ancestry components distinctive to for the Mikea were found, it is likely that they have adopted their hunter-gatherer way of life by through cultural reversion.

2013-12-19 | GSE53445 | GEO

AtLEGbeta / gamma protease specificity and putative substrate characterization by HUNTER

Project description:Arabidopsis thaliana Legumain (aka VPE) beta / gamma were characterized for their cleavage specificity and putative substrates were identified by the N-termini enrichment method HUNTER.

2020-07-29 | PXD019276 | Pride

Genome wide evidence of Austronesian-Bantu admixture and cultural reversion in a hunter-gatherer group of Madagascar

2013-12-19 | E-GEOD-53445 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data