Dataset Information

Modeling protein destiny in developing tomato fruit

ABSTRACT: Proteomics and transcriptomics data of tomato fruits at 9 developmental stages were used to calculate with a mathematical model the rate constants of synthesis and degradation for over 1,000 proteins.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Solanum Lycopersicum

TISSUE(S): Pericarp

SUBMITTER: Mélisande Blein-Nicolas

LAB HEAD: Sophie Colombié

PROVIDER: PXD012877 | Pride | 2019-05-06

REPOSITORIES: Pride

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Publications

Modeling Protein Destiny in Developing Fruit.

Belouah Isma I Nazaret Christine C Pétriacq Pierre P Prigent Sylvain S Bénard Camille C Mengin Virginie V Blein-Nicolas Mélisande M Denton Alisandra K AK Balliau Thierry T Augé Ségolène S Bouchez Olivier O Mazat Jean-Pierre JP Stitt Mark M Usadel Björn B Zivy Michel M Beauvoit Bertrand B Gibon Yves Y Colombié Sophie S

Plant physiology 20190423 3

Protein synthesis and degradation are essential processes that regulate cell status. Because labeling in bulky organs, such as fruits, is difficult, we developed a modeling approach to study protein turnover at the global scale in developing tomato (<i>Solanum lycopersicum</i>) fruit. Quantitative data were collected for transcripts and proteins during fruit development. Clustering analysis showed smaller changes in protein abundance compared to mRNA abundance. Furthermore, protein and transcrip ...[more]

PMID: 31015299

Publication: 1/2

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Project description:Transcriptomic analysis in response to Botrytis cinerea infections under conrasting nitrate regime Nitrogen (N) is one of the main limiting nutrients for plant growth and crop yield. It is well documented that changes in nitrate availability, the main N source found in agricultural soils, influences a myriad of developmental programs and processes including the plant defense response. Indeed, many agronomical reports indicate that the plant N nutritional status influences their ability to respond effectively when challenged by different pathogens. However, the molecular mechanisms involved in N-modulation of plant susceptibility to pathogens are poorly characterized. In this work, we show that Solanum lycopersicum defense response to the necrotrophic fungus Botrytis cinerea is affected by plant N availability, with higher susceptibility in nitrate-limiting conditions. Global gene expression responses of tomato against B. cinerea under contrasting nitrate conditions reveals that plant primary metabolism is affected by the fungal infection regardless of N regimes. This result suggests that differential susceptibility to pathogen attack under contrasting N conditions is not only explained by a metabolic alteration. We used a systems biology approach to identify the transcriptional regulatory network implicated in plant response to the fungus infection under contrasting nitrate conditions. Interestingly, hub genes in this network are known key transcription factors involved in ethylene and jasmonic acid signaling. This result positions these hormones as key integrators of nitrate and defense against B. cinerea in tomato plants. Our results provide insights into potential crosstalk mechanisms between necrotrophic defense response and N status in plants. To better understand the molecular changes underlying the impact of nitrate availability on plant susceptibility to B. cinerea, we performed plant transcriptome profiling assays on mock-treated (3 biological replicates) and infected plants ( 3 biological replicates) grown under three N conditions, using GeneChip Tomato Genome Arrays (Affymetrix).

Dataset Information

Modeling protein destiny in developing tomato fruit

Publications

Modeling Protein Destiny in Developing Fruit.

Similar Datasets

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