Proteomics

Dataset Information

4

MSCs OA SILAC Mass spectrometry imaging


ABSTRACT: In osteoarthritis (OA), impairment of cartilage regeneration can be related to a defective chondrogenic differentiation of mesenchymal stromal cells (MSCs). Therefore, understanding the proteomic- and metabolomic-associated molecular events during the chondrogenesis of MSCs could provide alternative targets for therapeutic intervention. Here, a SILAC-based proteomic analysis identified 43 proteins related with metabolic pathways whose abundance was significantly altered during the chondrogenesis of OA human bone marrow MSCs (hBMSCs). Then, the level and distribution of metabolites was analyzed in these cells and healthy controls by matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI), leading to the recognition of characteristic metabolomic profiles at the early stages of differentiation. Finally, integrative pathway analysis showed that UDP-glucuronic acid synthesis and amino sugar metabolism were downregulated in OA hBMSCs during chondrogenesis compared to healthy cells. Alterations in these metabolic pathways may disturb the production of hyaluronic acid (HA) and other relevant cartilage extracellular matrix (ECM) components. This work provides a novel integrative insight into the molecular alterations of osteoarthritic MSCs and potential therapeutic targets for OA drug development through the enhancement of chondrogenesis.

INSTRUMENT(S): solariX, 4800 Proteomics Analyzer

ORGANISM(S): Homo sapiens  

TISSUE(S): Bone Marrow

DISEASE(S): Osteoarthritis

SUBMITTER: Beatriz Rocha  

LAB HEAD: Francisco Javier Blanco García

PROVIDER: PXD012879 | Pride | 2020-01-27

REPOSITORIES: pride

altmetric image

Publications

Integrative Metabolic Pathway Analysis Reveals Novel Therapeutic Targets in Osteoarthritis.

Rocha Beatriz B   Cillero-Pastor Berta B   Eijkel Gert G   Calamia Valentina V   Fernandez-Puente Patricia P   Paine Martin R L MRL   Ruiz-Romero Cristina C   Heeren Ron M A RMA   Blanco Francisco J FJ  

Molecular & cellular proteomics : MCP 20200124 4


In osteoarthritis (OA), impairment of cartilage regeneration can be related to a defective chondrogenic differentiation of mesenchymal stromal cells (MSCs). Therefore, understanding the proteomic- and metabolomic-associated molecular events during the chondrogenesis of MSCs could provide alternative targets for therapeutic intervention. Here, a SILAC-based proteomic analysis identified 43 proteins related with metabolic pathways whose abundance was significantly altered during the chondrogenesis  ...[more]

Similar Datasets

2016-02-05 | E-GEOD-63745 | ArrayExpress
2015-06-30 | E-GEOD-64393 | ArrayExpress
2015-06-30 | E-GEOD-64394 | ArrayExpress
2014-02-21 | E-GEOD-55235 | ArrayExpress
2018-10-24 | PXD002014 | Pride
1000-01-01 | S-EPMC4529975 | BioStudies
2015-07-14 | E-GEOD-70856 | ArrayExpress
2019-03-20 | E-GEOD-55235 | ExpressionAtlas
1000-01-01 | S-EPMC5935742 | BioStudies
1000-01-01 | S-EPMC6305125 | BioStudies