Proteomics

Dataset Information

0

Alterations of redox and iron metabolism accompany development of HIV latency

ABSTRACT: Metabolic alterations, such as oxidative stress, are hallmarks of HIV-1 infection. However, their influenceon the development of viral latency, and thus on HIV-1 persistence during antiretroviral therapy (ART),have just begun to be explored. We analyzed omics profiles ofin-vitroandin-vivomodels of infection byHIV-1 and its simian homolog SIVmac. We found that cells survive retroviral replication by upregulatingantioxidant pathways and intertwined iron import pathways. These changes are associated withremodeling of the redox sensitive promyelocytic leukemia protein nuclear bodies (PML NBs), an importantconstituent of nuclear architecture and a marker of HIV-1 latency. We found that PML is depleted inproductively infected cells and restored by ART. Moreover, we identified intracellular iron as a key linkbetween oxidative stress and PML depletion, thus supporting iron metabolism modulators aspharmacological tools to impair latency establishment.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Christian Frese  

LAB HEAD: Marina Lusic

PROVIDER: PXD012907 | Pride | 2020-03-03

REPOSITORIES: Pride

Json Xml
altmetric image

Publications

HIV-1 persists in a latent form during antiretroviral therapy, mainly in CD4<sup>+</sup> T cells, thus hampering efforts for a cure. HIV-1 infection is accompanied by metabolic alterations, such as oxidative stress, but the effect of cellular antioxidant responses on viral replication and latency is unknown. Here, we show that cells survive retroviral replication, both in vitro and in vivo in SIVmac-infected macaques, by upregulating antioxidant pathways and the intertwined iron import pathway.  ...[more]

Similar Datasets

Unknown Alterations of redox and iron metabolism accompany development of HIV latency
2020-01-30 | GSE127468 | GEO
Unknown Transcriptional response of HIV-infected CD4 memory T cells to co-culture with TCR activated CD8 T cells.
2023-04-27 | GSE198525 | GEO
Unknown HIV latency reversal via combined non-canonical NF- B agonism and targeted BET family bromodomain inhibition
2022-02-10 | GSE196091 | GEO
Unknown Aminobisphosphonates reactivate the latent reservoir in people living with HIV-1
2023-09-13 | GSE224546 | GEO
Unknown Transcriptome analysis in primary cell models of HIV latency and cells from HIV-infected ART-suppressed individuals
2020-10-28 | GSE144585 | GEO
Unknown A histone deacetylase network regulates epigenetic reprogramming and viral silencing in HIV infected cells
2023-08-08 | GSE199946 | GEO
Unknown Memory CD4+ T cell subsets show differential responses to HIV latency reversing agents [LRA stimulated]
2018-01-26 | GSE94150 | GEO
Unknown Memory CD4+ T cell subsets show differential responses to HIV latency reversing agents [LARA]
2018-01-26 | GSE94149 | GEO
Unknown Systemic HIV/SIV latency reversal via non-canonical NF-κB signaling in vivo
2020-01-11 | GSE142774 | GEO
Transcriptomics Potent and targeted activation of HIV-1 using the CRISPR/Cas9 activator Complex
2016-08-20 | E-GEOD-72259 | biostudies-arrayexpress