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Quantitative proteomic analysis of heat shock response using tandem mass tags

ABSTRACT: Analysis of precision of TMT quantitation at the peptide level using multinotch MS3

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: Aaron Storey

LAB HEAD: Jeffrey Lewis

PROVIDER: PXD012973 | Pride | 2020-02-18

REPOSITORIES: Pride

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Accurate and Sensitive Quantitation of the Dynamic Heat Shock Proteome Using Tandem Mass Tags.

Storey Aaron J AJ Hardman Rebecca E RE Byrum Stephanie D SD Mackintosh Samuel G SG Edmondson Rick D RD Wahls Wayne P WP Tackett Alan J AJ Lewis Jeffrey A JA

Journal of proteome research 20200219 3

Cells respond to environmental perturbations and insults through modulating protein abundance and function. However, the majority of studies have focused on changes in RNA abundance because quantitative transcriptomics has historically been more facile than quantitative proteomics. Modern Orbitrap mass spectrometers now provide sensitive and deep proteome coverage, allowing direct, global quantification of not only protein abundance but also post-translational modifications (PTMs) that regulate ...[more]

PMID: 32027144

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