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Proteomic analysis of endometrioid and high-grade serous ovarian tumour samples

ABSTRACT: Precision medicine approaches in ovarian cancer require the accurate diagnosis of histotypes. In particular, there is a critical need to distinguish endometroid (EC) from high grade serous (HGSC) carcinomas, which differ greatly in outcomes, genetic predisposition and optimal treatment approaches. Herein, we performed label-free quantitative proteomics on freshly frozen tumour tissues to discover biomarkers that may distinguish EC from HGSC, and then used IHC to validate these using a contemporarily classified cohort of EC and HGSC samples.

INSTRUMENT(S): Exactive Plus, LTQ Orbitrap Elite

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Ovary, Ovary Cancer Cell

DISEASE(S): Ovarian Cancer,Ovarian Serous Carcinoma,Endometrioid Ovary Carcinoma

SUBMITTER: Dylan Dieters-Castator

LAB HEAD: Gilles A. Lajoie

PROVIDER: PXD012998 | Pride | 2019-03-13

REPOSITORIES: Pride

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Proteomics-Derived Biomarker Panel Improves Diagnostic Precision to Classify Endometrioid and High-grade Serous Ovarian Carcinoma.

Dieters-Castator Dylan Z DZ Rambau Peter F PF Kelemen Linda E LE Siegers Gabrielle M GM Lajoie Gilles A GA Postovit Lynne-Marie LM Köbel Martin M

Clinical cancer research : an official journal of the American Association for Cancer Research 20190412 14

<h4>Purpose</h4>Ovarian carcinomas are a group of distinct diseases classified by histotypes. As histotype-specific treatment evolves, accurate classification will become critical for optimal precision medicine approaches.<h4>Experimental design</h4>To uncover differences between the two most common histotypes, high-grade serous (HGSC) and endometrioid carcinoma, we performed label-free quantitative proteomics on freshly frozen tumor tissues (HGSC, <i>n</i> = 10; endometrioid carcinoma, <i>n</i> ...[more]

PMID: 30979743

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