Dataset Information

LC-MSMS of HSV-1 infected HFFs

ABSTRACT: Herpes simplex virus 1 (HSV-1) is the causative agent of the common cold sores and life-threatening disease. Here, we characterize the full complement of viral gene expression during lytic infection using an integrative Omics approach. This revealed hundreds of novel viral gene products arising from differential usage of transcription and translation start sites. We classify these into viral gene modules and provide a comprehensive state-of-the-art annotation of all HSV-1 gene products.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Homo Sapiens (human) Human Herpesvirus 1 (strain 17) (hhv-1) (human Herpes Simplex Virus 1)

TISSUE(S): Fibroblast

DISEASE(S): Disease Free

SUBMITTER: Florian Erhard

LAB HEAD: Lars Dölken

PROVIDER: PXD013010 | Pride | 2020-05-22

REPOSITORIES: Pride

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Publications

Integrative functional genomics decodes herpes simplex virus 1.

Whisnant Adam W AW Jürges Christopher S CS Hennig Thomas T Wyler Emanuel E Prusty Bhupesh B Rutkowski Andrzej J AJ L'hernault Anne A Djakovic Lara L Göbel Margarete M Döring Kristina K Menegatti Jennifer J Antrobus Robin R Matheson Nicholas J NJ Künzig Florian W H FWH Mastrobuoni Guido G Bielow Chris C Kempa Stefan S Liang Chunguang C Dandekar Thomas T Zimmer Ralf R Landthaler Markus M Grässer Friedrich F Lehner Paul J PJ Friedel Caroline C CC Erhard Florian F Dölken Lars L

Nature communications 20200427 1

The predicted 80 open reading frames (ORFs) of herpes simplex virus 1 (HSV-1) have been intensively studied for decades. Here, we unravel the complete viral transcriptome and translatome during lytic infection with base-pair resolution by computational integration of multi-omics data. We identify a total of 201 transcripts and 284 ORFs including all known and 46 novel large ORFs. This includes a so far unknown ORF in the locus deleted in the FDA-approved oncolytic virus Imlygic. Multiple transcr ...[more]

PMID: 32341360

Dataset Information

LC-MSMS of HSV-1 infected HFFs

Publications

Integrative functional genomics decodes herpes simplex virus 1.

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