Dataset Information

Comparison of Amphibalanus amphitrite exuviae (molts) and adhesive

ABSTRACT: The base of the barnacle Amphibalanus amphitrite has a layer of protein and cuticle that adheres the barnacle to the surface. The proteins in the adhesive have been characterized, but it is unknown whether the adhesive proteins are associated with the cuticle. To address this possibility, we examined the proteins associated with the molt (exuviae) from the main body, which is also composed of cuticle.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Amphibalanus Amphitrite (striped Barnacle) (balanus Amphitrite)

SUBMITTER: Janna Schultzhaus

LAB HEAD: Janna Schultzhaus

PROVIDER: PXD013036 | Pride | 2021-09-08

REPOSITORIES: Pride

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Publications

Adhesion of acorn barnacles on surface-active borate glasses.

Fears Kenan P KP Barnikel Andrew A Wassick Ann A Ryou Heonjune H Schultzhaus Janna N JN Orihuela Beatriz B Scancella Jenifer M JM So Christopher R CR Hunsucker Kelli Z KZ Leary Dagmar H DH Swain Geoffrey G Rittschof Daniel D Spillmann Christopher M CM Wahl Kathryn J KJ

Philosophical transactions of the Royal Society of London. Series B, Biological sciences 20190909 1784

Concerns about the bioaccumulation of toxic antifouling compounds have necessitated the search for alternative strategies to combat marine biofouling. Because many biologically essential minerals have deleterious effects on organisms at high concentration, one approach to preventing the settlement of marine foulers is increasing the local concentration of ions that are naturally present in seawater. Here, we used surface-active borate glasses as a platform to directly deliver ions (Na<sup>+</sup ...[more]

PMID: 31495306

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Project description:Background: Marine biofouling negatively impacts industries reliant upon submerged stationary surfaces. The acorn barnacle Amphibalanus amphitrite is a major biofouler that permanently attaches to a wide array of substrates by producing a proteinaceous adhesive at the surface interface. These interface proteins are difficult to solubilize, and the understanding of what proteins exist in the adhesive has only been expanded with the use of the strong polar solvent hexafluoroisopropanol in combination with standard gel-based sample processing methods for proteomics analysis. Although effective, existing sample processing methods are labor and time intensive, hindering progress in this field. Results: We have developed a more efficient sample processing method by exploiting pressure cycling technology, which aids in protein extraction and digestion for proteomics analysis, and explored the efficacy of multiple solvents in combination with pressure on protein identification. We found that barnacle adhesive proteins can be extracted and digested in the same tube using pressure cycling technology, minimizing sample loss, increasing throughput to 16 concurrently processed samples, and decreasing sample processing time to under 8 hours. Pressure cycling technology methods produced similar proteomes in comparison to previous methods. Two solvents which were ineffective at extracting proteins from the adhesive at ambient pressure (urea and methanol) produced more protein identifications under pressure than hexafluoroisopropanol, leading to the identification and our description of >40 novel proteins at the interface. Many A. amphitrite adhesive proteins have no sequence similarity to publically available proteins, highlighting the unique adherent processes evolved by barnacles. Conclusion: Using pressure cycling technology, we describe methods that produce robust and consistent protein identifications from barnacle adhesive. These methods can be used to examine changes in barnacle adhesive, enabling future research on the effects of environmental and surface substrate composition on barnacle cement, a critical step for both broadening the understanding of barnacle adhesion and the development of new coatings for submerged surfaces to combat hard biofoulants.

Dataset Information

Comparison of Amphibalanus amphitrite exuviae (molts) and adhesive

Publications

Adhesion of acorn barnacles on surface-active borate glasses.

Similar Datasets

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