Proteomic analysis of 3D cultures of fibroblasts and Ras-transformed HaCaT keratinocytes

ABSTRACT: HaCaT, and its three retrovirally c-Ha-ras-transformed subclones, A5, II-4 and RT3 (abbreviated as ras-HaCaTs in the text) that exhibit benign, invasive and malignant phenotypes, respectively, were cultured as 3D monocultures and co-cultures with fibroblasts. After hypotonic lysis of the cells, the insoluble proteins were analysed by LC-MS/MS.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Keratinocyte, Fibroblast

SUBMITTER: Pekka Rappu  

LAB HEAD: Jyrki Heino

PROVIDER: PXD013113 | Pride | 2019-09-10

REPOSITORIES: Pride