Dataset Information

Asparagine hydroxylation is likely to be a reversible post-translational modification

ABSTRACT: Amino acid hydroxylation is a common post-translational modification, which generally regulates protein interactions or adds a functional group that can be further modified. Such hydroxylation is currently considered irreversible, necessitating the degradation and re-synthesis of the entire protein to reset the modification. Here we present evidence that the cellular machinery can reverse FIH-mediated asparagine hydroxylation on intact proteins. These data suggest that asparagine hydroxylation is a flexible and dynamic post-translational modification akin to modifications involved in regulating signalling networks, such as phosphorylation, methylation and ubiquitylation.

INSTRUMENT(S):

ORGANISM(S): Hordeum Vulgare (barley)

TISSUE(S): Epithelial Cell

SUBMITTER: Alex von kriegsheim