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Pillar Array Columns for Peptide Separations

ABSTRACT: Reversed-phase chromatography is the most common technique for separation of tryptic peptides. In this manuscript, we describe the optimization of sample loading and separation parameters for a novel micro machined column and provide a detailed description on the performance and reproducibility of this separation system.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Hela Cell

SUBMITTER: Goran Mitulović

LAB HEAD: Goran Mitulović

PROVIDER: PXD013158 | Pride | 2019-07-18

REPOSITORIES: Pride

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Pillar array columns for peptide separations in nanoscale reversed-phase chromatography.

Tóth Gábor G Panić-Janković Tanja T Mitulović Goran G

Journal of chromatography. A 20190704

Reversed-phase chromatography is the most common technique for separation of tryptic peptides. In this short communication, we describe the optimization of sample loading and separation parameters for a novel micromachined column and provide a detailed description on the performance and reproducibility of this separation system. Tryptic digest of a mixture of seven proteins with diverse mass and isoelectric point was used as a test sample. The methods developed and used are straight-forward; by ...[more]

PMID: 31301798

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