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DUB Profiling of 20 different breast cancer cell lines.

ABSTRACT: De-ubiquitinating enzymess from 20 Different breast cancer cell lines were purified using an Affinity Binding Probe.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

DISEASE(S): Breast Cancer

SUBMITTER: Román González-Prieto

LAB HEAD: Alfred C.O. Vertegaal

PROVIDER: PXD013161 | Pride | 2020-01-29

REPOSITORIES: Pride

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Deubiquitinase Activity Profiling Identifies UCHL1 as a Candidate Oncoprotein That Promotes TGFβ-Induced Breast Cancer Metastasis.

Liu Sijia S González-Prieto Román R Zhang Mengdi M Geurink Paul P PP Kooij Raymond R Iyengar Prasanna Vasudevan PV van Dinther Maarten M Bos Erik E Zhang Xiaobing X Le Dévédec Sylvia E SE van de Water Bob B Koning Roman I RI Zhu Hong-Jian HJ Mesker Wilma E WE Vertegaal Alfred C O ACO Ovaa Huib H Zhang Long L Martens John W M JWM Ten Dijke Peter P

Clinical cancer research : an official journal of the American Association for Cancer Research 20191219 6

<h4>Purpose</h4>Therapies directed to specific molecular targets are still unmet for patients with triple-negative breast cancer (TNBC). Deubiquitinases (DUB) are emerging drug targets. The identification of highly active DUBs in TNBC may lead to novel therapies.<h4>Experimental design</h4>Using DUB activity probes, we profiled global DUB activities in 52 breast cancer cell lines and 52 patients' tumor tissues. To validate our findings <i>in vivo</i>, we employed both zebrafish and murine breast ...[more]

PMID: 31857432

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