Ontology highlight
ABSTRACT:
INSTRUMENT(S): impact HD
ORGANISM(S): Homo Sapiens (human) Mus Musculus (mouse)
TISSUE(S): Kidney
DISEASE(S): Chromophobe Renal Cell Carcinoma,Renal Oncocytoma
SUBMITTER: Hugo M. Santos
LAB HEAD: Hugo M. Santos
PROVIDER: PXD013164 | Pride | 2019-06-13
REPOSITORIES: Pride
Jorge Susana S Capelo José L JL LaFramboise William W Dhir Rajiv R Lodeiro Carlos C Santos Hugo M HM
Journal of proteome research 20190619 7
An effective three-step proteomics workflow is proposed to overcome the pitfalls caused by polymers present in optimum cutting temperature (OCT)-embedded tissue during its preparation for mass spectrometry analysis. First, the OCT-embedded tissue biopsies are cleaned using ethanol and water in a sequential series of ultrasonic washes in an ultrasound bath (35 kHz ultrasonic frequency, 100% ultrasonic amplitude, 2 min of ultrasonic duty time). Second, a fast ultrasonic-assisted extraction of prot ...[more]