Tracking genome-editing and associated molecular perturbations by SWATH mass spectrometry

ABSTRACT: Advances in gene editing now allow reverse genetics to be applied to a broad range of biological systems. Ultimately, any modification to coding sequences requires confirmation at the protein level, although immunoblotting is often hampered by antibody quality or availability especially in non-mammalian species. Here, we show that Sequential Window Acquisition of All Theoretical Spectra (SWATH) - Mass Spectrometry (MS) is a robust antibody-independent alternative for monitoring gene editing at the protein level and concomitantly defines proteome responses which may provide pertinent biological insights.

INSTRUMENT(S):

ORGANISM(S): Bicyclus Anynana Danio Rerio (zebrafish) (brachydanio Rerio) Mus Musculus (mouse)

TISSUE(S): Pupa, Embryo, Tail Fin, Cell Culture

SUBMITTER: Qifeng Lin  

LAB HEAD: Jayantha Gunaratne

PROVIDER: PXD013176 | Pride | 2019-10-24

REPOSITORIES: Pride