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S-acylproteomic analysis of human prostate cancer LNCaP cells by LB-ABE

ABSTRACT: Deep S-acylproteomic analysis of LNCaP cells by coupling LB-ABE with label-free quantitative proteomics

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell

DISEASE(S): Prostate Adenocarcinoma

SUBMITTER: Wei Yang

LAB HEAD: Wei Yang

PROVIDER: PXD013187 | Pride | 2021-09-08

REPOSITORIES: Pride

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Low-Background Acyl-Biotinyl Exchange Largely Eliminates the Coisolation of Non-S-Acylated Proteins and Enables Deep S-Acylproteomic Analysis.

Zhou Bo B Wang Yang Y Yan Yiwu Y Mariscal Javier J Di Vizio Dolores D Freeman Michael R MR Yang Wei W

Analytical chemistry 20190711 15

Protein S-acylation (also called palmitoylation) is a common post-translational modification whose deregulation plays a key role in the pathogenesis of many diseases. Acyl-biotinyl exchange (ABE), a widely used method for the enrichment of S-acylated proteins, has the potential of capturing the entire S-acylproteome in any type of biological sample. Here, we showed that current ABE methods suffer from a high background arising from the coisolation of non-S-acylated pr ...[more]

PMID: 31251020

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