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LC-MS/MS analyses of MMP cleavage fragments of tyrosyl-tRNA synthetase

ABSTRACT: Tyrosyl-tRNA synthetase was digested with MMPs after which cleavage fragments were resolved using SDS-PAGE. Major bands were digested with trypsin and analyzed by LC-MS/MS.

INSTRUMENT(S): impact II

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Parker Jobin

LAB HEAD: Christopher Mark Overall

PROVIDER: PXD013197 | Pride | 2019-05-30

REPOSITORIES: Pride

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Gel LC-MS/MS analyses of MMP cleavage of tryptophanyl-tRNA synthetase

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ATOMS analyses of MMP cleavage sites in WRS

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Comparative genomic hybridization of B. pertussis chromosomal fragments separated by PFGE

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Transcription profiling of mouse C57Bl6 MMP-7 and MMP-10 deficient mice Pseudomonas aeruginosa exposed airway epithelium.

Project description:Airway epithelium is the initial point of host-pathogen interaction in Pseudomonas aeruginosa infection, an important pathogen in cystic fibrosis and nosocomial pneumonia. We used global gene expression analysis to determine airway epithelial transcriptional responses dependent on matrilysin (MMP-7) and stromelysin-2 (MMP-10), two matrix metalloproteinases induced by acute P. aeruginosa pulmonary infection. Extraction of Differential Gene Expression (EDGE) analysis of gene expression changes in P. aeruginosa infected organotypic tracheal epithelial cell cultures from wildtype, Mmp7-/-, and Mmp10-/- mice identified 2,089 matrilysin-dependent and 1,628 stromelysin-2-dependent genes that were differentially expressed. Key node network analysis showed that these MMPs controlled distinct gene expression programs involved in proliferation, cell death, immune responses, and signal transduction, among other host defense processes. Our results demonstrate discrete roles for these MMPs in regulating epithelial responses to pseudomonas infection and show that a global genomics strategy can be used to assess MMP function. Experiment Overall Design: C57Bl6, Mmp7-/- and Mmp10-/- mouse epithelium at an organotypic air liquid interface culture was exposed to Pseudomonas aeruginosa for 1 and 24 h. RNA was collected from these samples as well as uninfected 0 h and assessed for expression changes using Affymetrix Mouse 430 2.0 Arrays. Triplicate samples were processed for each genotype at each time point.

2008-06-15 | E-GEOD-7957 | biostudies-arrayexpress

Expression data from Pseudomonas aeruginosa exposed airway epithelium from C57Bl6 and MMP-7 and MMP-10 deficient mice.

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CELF1 upregulates MMP gene expression to regulate cataract development

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MiR-21 Promotes Glioma Invasion by Targeting MMP Regulators.

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2015-09-29 | PXD002265 | Pride

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