Rational Design of High Affinity Peptides Validates Histone Chaperone ASF1 as a New Epigenetic Target in Cancer

ABSTRACT: Starting from the structure of the human ASF1-histone complex, we developed a rational design strategy combining epitope tethering and optimization of interface contacts to identify a potent peptide inhibitor with a dissociation constant of 3 nM. LC-MS/MS was used to identify in vivo targets of the most prominent peptide inhibitor.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Uterine Cervix

DISEASE(S): Cervical Adenocarcinoma

SUBMITTER: Valentin SABATET  

LAB HEAD: Damarys Loew

PROVIDER: PXD013279 | Pride | 2019-09-25

REPOSITORIES: Pride