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Mass Spectrometry analysis of a Arabidopsis protein

ABSTRACT: We identified a Arabidopsis protein BSF, and it has a role in the stabilization and translation of chloroplast mRNAs. To further study it's function, we purified BSF-associated proteins for mass spectrometry analysis.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Leaf

SUBMITTER: Xin Chai

LAB HEAD: Wei Chi

PROVIDER: PXD013325 | Pride | 2019-04-30

REPOSITORIES: Pride

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Publications

An RNA Chaperone-Like Protein Plays Critical Roles in Chloroplast mRNA Stability and Translation in Arabidopsis and Maize.

Jiang Jingjing J Chai Xin X Manavski Nikolay N Williams-Carrier Rosalind R He Baoye B Brachmann Andreas A Ji Daili D Ouyang Min M Liu Yini Y Barkan Alice A Meurer Jörg J Zhang Lixin L Chi Wei W

The Plant cell 20190408 6

A key characteristic of chloroplast gene expression is the predominance of posttranscriptional control via numerous nucleus-encoded RNA binding factors. Here, we explored the essential roles of the S1-domain-containing protein photosynthetic electron transfer B (petB)/ petD Stabilizing Factor (BSF) in the stabilization and translation of chloroplast mRNAs. BSF binds to the intergenic region of petB-petD, thereby stabilizing 3' processed petB transcripts ...[more]

PMID: 30962391

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