Dataset Information

Compositional evolution of centipede venom

ABSTRACT: This project provides the first insight and comparative data into the venoms of all five orders of centipede

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Craterostigmus Tasmanianus Lithobius Forficatus Scutigera Coleoptrata Strigamia Maritima Scolopendra Morsitans

TISSUE(S): Venom

SUBMITTER: Eivind Undheim

LAB HEAD: Eivind Andreas Baste Undheim

PROVIDER: PXD013356 | Pride | 2020-05-06

REPOSITORIES: Pride

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Publications

Parallel Evolution of Complex Centipede Venoms Revealed by Comparative Proteotranscriptomic Analyses.

Jenner Ronald A RA von Reumont Bjoern M BM Campbell Lahcen I LI Undheim Eivind A B EAB

Molecular biology and evolution 20191201 12

Centipedes are among the most ancient groups of venomous predatory arthropods. Extant species belong to five orders, but our understanding of the composition and evolution of centipede venoms is based almost exclusively on one order, Scolopendromorpha. To gain a broader and less biased understanding we performed a comparative proteotranscriptomic analysis of centipede venoms from all five orders, including the first venom profiles for the orders Lithobiomorpha, Craterostigmomorpha, and Geophilom ...[more]

PMID: 31396628

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Project description:Spider venoms are a unique source of bioactive peptides displaying remarkable structural stability and potent neuroactivity. Phoneutria nigriventer, often referred to as Brazilian wandering spider, banana spider or “armed” spider, is endemic from South America and amongst the most dangerous venomous spiders in the world. Envenomation accidents with P. nigriventer often occur in Brazil with approximately 4,000 cases per year and which symptoms include priapism, hypertension, blurred vision, sweating, and vomiting, amongst others. Besides its clinical relevance, P. nigriventer venom comprises promising peptide drug leads providing therapeutic effects in a range of disease models. In this study, we further explored the neuroactivity and molecular diversity of the venom from P. nigriventer using fractionation-guided high-throughput cellular assays coupled to proteomics analysis and multi-pharmacology activity to broaden the knowledge and therapeutic potential of this venom, as well as a proof-of-concept for an investigative pipeline to study spider-venom derived neuroactive peptides. We applied ion channel assays in a neuroblastoma cell line to investigate modulation of voltage-gated sodium and calcium channels, and nicotinic acetylcholine α7 receptor. We then investigated the venom components using mass spectrometry to identify peptide masses and sequences associated to the observed neuromodulations. Our findings showed this venom is highly complex compared to other neurotoxin-rich venoms and comprises potent modulators of voltage-gated sodium and calcium channels which were classified into 4 families of neuroactive peptides based on their activities and structures. In addition to the reported P. nigriventer neuroactive peptides, we identified at least 27 novel cysteine-rich venom peptides in which neuroactivities are still to be elucidated in voltage-gated ion channels and other potential targets. Our findings provide a new basis for studying non-explored bioactivities of known and novel neuroactive components in P. nigriventer venom, and further supports the successful application of our discovery pipeline for identifying ion channel-targeting venom peptides with potential to become drug leads with diverse exploratory and therapeutic applications.

Dataset Information

Compositional evolution of centipede venom

Publications

Parallel Evolution of Complex Centipede Venoms Revealed by Comparative Proteotranscriptomic Analyses.

Similar Datasets

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