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Cerebellum Proteomics - Cell-type-specific profiling of brain mitochondria reveals functional and molecular diversity

ABSTRACT: A whole cerebellum proteome analysis was performed to compare the data to cell specific mitochondria proteomics in the same brain region.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Brain

SUBMITTER: Stephan Mueller

LAB HEAD: Thomas Misgeld

PROVIDER: PXD013380 | Pride | 2019-09-03

REPOSITORIES: Pride

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			Action	DRS
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	Cer_2.raw	Raw
	Cer_3.raw	Raw
	Cer_4.raw	Raw
	MaxQuant_Output.zip	Other

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Publications

Cell-type-specific profiling of brain mitochondria reveals functional and molecular diversity.

Fecher Caroline C Trovò Laura L Müller Stephan A SA Snaidero Nicolas N Wettmarshausen Jennifer J Heink Sylvia S Ortiz Oskar O Wagner Ingrid I Kühn Ralf R Hartmann Jana J Karl Rosa Maria RM Konnerth Arthur A Korn Thomas T Wurst Wolfgang W Merkler Doron D Lichtenthaler Stefan F SF Perocchi Fabiana F Misgeld Thomas T

Nature neuroscience 20190909 10

Mitochondria vary in morphology and function in different tissues; however, little is known about their molecular diversity among cell types. Here we engineered MitoTag mice, which express a Cre recombinase-dependent green fluorescent protein targeted to the outer mitochondrial membrane, and developed an isolation approach to profile tagged mitochondria from defined cell types. We determined the mitochondrial proteome of the three major cerebellar cell types (Purkinje cells, granule cells and as ...[more]

PMID: 31501572

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