Proteomics

Dataset Information

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Photosystem II assembly No-reaction center (NRC) complex


ABSTRACT: LC-MS/MS identification of small subunit peptides of Photosystem II assembly intermediate (NRC) with/without trypsin digestion. Peptide sequence identification with HCD fragmentation using Q-Exactive plus mass spectrometer (Thermo-Fisher)

INSTRUMENT(S): Q Exactive

ORGANISM(S): Synechocystis Sp. Pcc 6803

TISSUE(S): Photosynthetic Cell, Cell Culture

SUBMITTER: Haijun Liu  

LAB HEAD: Himadri B Pakrasi

PROVIDER: PXD013406 | Pride | 2019-10-14

REPOSITORIES: Pride

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Publications

A novel chlorophyll protein complex in the repair cycle of photosystem II.

Weisz Daniel A DA   Johnson Virginia M VM   Niedzwiedzki Dariusz M DM   Shinn Min Kyung MK   Liu Haijun H   Klitzke Clécio F CF   Gross Michael L ML   Blankenship Robert E RE   Lohman Timothy M TM   Pakrasi Himadri B HB  

Proceedings of the National Academy of Sciences of the United States of America 20191008 43


In oxygenic photosynthetic organisms, photosystem II (PSII) is a unique membrane protein complex that catalyzes light-driven oxidation of water. PSII undergoes frequent damage due to its demanding photochemistry. It must undergo a repair and reassembly process following photodamage, many facets of which remain unknown. We have discovered a PSII subcomplex that lacks 5 key PSII core reaction center polypeptides: D1, D2, PsbE, PsbF, and PsbI. This pigment-protein complex does contain the PSII core  ...[more]

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