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Proteomics analysis of the Enterobacter cloacae response to imipenem

ABSTRACT: Recently, we have reported on a highly drug-resistant carbapenemase-producing isolate of Enterobacter cloacae (Nepal et al., Virulence. 2018; 9: 1377-1389). In the present study, we asked the question whether and, if so, how this isolate responds to a sub-inhibitory challenge with the antibiotic imipenem. To answer this question, we applied a SILAC proteomics approach that allowed the quantification of changes in the relative abundance of bacterial protein in response to imipenem. The results show that the investigated E. cloacae isolate mounts a highly specific response to counteract the detrimental effects of imipenem.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Enterobacter Cloacae

SUBMITTER: Sandra Maass

LAB HEAD: Dörte Becher

PROVIDER: PXD013412 | Pride | 2021-08-10

REPOSITORIES: Pride

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Proteomic Charting of Imipenem Adaptive Responses in a Highly Carbapenem Resistant Clinical Enterobacter roggenkampii Isolate.

Nepal Suruchi S Maaß Sandra S Grasso Stefano S Cavallo Francis M FM Bartel Jürgen J Becher Dörte D Bathoorn Erik E van Dijl Jan Maarten JM

Antibiotics (Basel, Switzerland) 20210428 5

Gram-negative bacteria belonging to the Enterobacter cloacae complex are increasingly implicated in difficult-to-treat nosocomial infections, as exemplified by a recently characterized highly carbapenem-resistant clinical Enterobacter roggenkampii isolate with sequence type (ST) 232. While mechanisms of carbapenem resistance are well-understood, little is known about the responses of highly drug-resistant bacteria to these antibiotics. Our present study was therefore aimed at chart ...[more]

PMID: 33924830

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