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The effect of very-high-molecular-mass hyaluronan (vHMM-HA) on CD44 protein-protein interactions.

ABSTRACT: Naked mole-rat skin fibroblasts (NSF) produces vHMM-HA. Although NSF-HA has been shown to suppress NSF transformation, it is still unclear if exceptional polymer length of vHMM-HA is important for its functions. Here, we compared the effect of intact control NSF-HA (cNSF-HA) and partially fragmented NSF-HA (fNSF-HA) on CD44 protein-protein interactions.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture, Fibroblast

DISEASE(S): Disease Free

SUBMITTER: Masaki Takasugi

LAB HEAD: Vera Gorbunova / Andrei Seluanov

PROVIDER: PXD013445 | Pride | 2020-03-24

REPOSITORIES: Pride

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Naked mole-rat very-high-molecular-mass hyaluronan exhibits superior cytoprotective properties.

Takasugi Masaki M Firsanov Denis D Tombline Gregory G Ning Hanbing H Ablaeva Julia J Seluanov Andrei A Gorbunova Vera V

Nature communications 20200512 1

Naked mole-rat (NMR), the longest-living rodent, produces very-high-molecular-mass hyaluronan (vHMM-HA), compared to other mammalian species. However, it is unclear if exceptional polymer length of vHMM-HA is important for longevity. Here, we show that vHMM-HA (>6.1 MDa) has superior cytoprotective properties compared to the shorter HMM-HA. It protects not only NMR cells, but also mouse and human cells from stress-induced cell-cycle arrest and cell death in a polymer length-dependent manner. The ...[more]

PMID: 32398747

Similar Datasets

The effect of very-high-molecular-mass hyaluronan (vHMM-HA) on CD44-overexpressing IMR90 cells

Project description:Naked mole-rat skin fibroblasts (NSF) produces vHMM-HA. Although NSF-HA has been shown to suppress NSF transformation, it is still unclear if exceptional polymer length of vHMM-HA is important for its functions. Here, we compared the effect of intact control NSF-HA (cNSF-HA) ) on the transcriptome of CD44-overexpressing IMR90 cells.

2020-04-25 | E-MTAB-8943 | biostudies-arrayexpress

The effect of very-high-molecular-mass hyaluronan (vHMM-HA) on IMR90 transcriptome

Project description:Naked mole-rat skin fibroblasts (NSF) produces vHMM-HA. Although NSF-HA has been shown to suppress NSF transformation, it is still unclear if exceptional polymer length of vHMM-HA is important for its functions. Here, we compared the effect of intact control NSF-HA (cNSF-HA) and partially fragmented NSF-HA (fNSF-HA) on the transcriptome of IMR90 cells. Related RNA-Seq datasets are deposited in ArrayExpress (E-MTAB-8943).

2019-01-01 | GSE116759 | GEO

Increased hyaluronan by naked mole-rat HAS2 extends healthspan in mice

Project description:Abundant high molecular weight hyaluronic acid (HMW-HA) contributes to cancer resistance and possibly longevity of the longest-lived rodent, the naked mole-rat1,2. To study whether the benefits of increased HMW-HA could be transferred to other animal species, we generated a transgenic mouse overexpressing naked mole-rat hyaluronic acid synthase 2 gene (nmrHAS2). nmrHAS2 mice showed increase in hyaluronan levels in several tissues, and lower incidence of spontaneous and induced cancer, extended lifespan and improved healthspan. The transcriptome signature of nmrHAS2 mice shifted towards that of longer-lived species. The most striking change observed in nmrHAS2 mice was attenuated inflammation across multiple tissues. HMW-HA reduced inflammation via several pathways including direct immunoregulatory effect on immune cells, protection from oxidative stress, and improved gut barrier function during aging. These findings demonstrate that the longevity mechanism that evolved in the naked mole-rat can be exploited to other species, and open new avenues for using HMW-HA to improve lifespan and healthspan

2023-09-08 | GSE234154 | GEO

Increased hyaluronan by naked mole-rat HAS2 extends healthspan in mice

2023-08-10 | GSE234563 | GEO

Increased hyaluronan by naked mole-rat HAS2 improves healthspan in mice [microbiome]

2023-06-12 | GSE234286 | GEO

Single-cell RNAseq of HA-specific CD8 T cells isolated from the hypothalamus and the spleen of mice expressing the model antigen HA in orexinergic neurons

Project description:We previously generated the Orex-HA mouse model in which orexin-producing neurons residing in the hypothalamus selectively expressed a model self-antigen, namely hemagglutinin (HA) from the H1N1 influenza virus. In this model the adoptive transfer of in vitro activated HA-specific CD8 T cells leads to the specific loss of orexinergic neurons located in the hypothalamus. To assess the phenotype and investigate the pathogenic mechanisms and the potential tissue-resident properties of autoreactive (HA-specific) CD8 T cells infiltrating the hypothalamus of Orex-HA mice , we performed a single-cell transcriptomic analysis (scRNA-seq) of HA-specific CD8+ T cells isolated from the hypothalamus and the spleen at day 30 after T cell transfer in Orex-HA mice.

2022-04-14 | E-MTAB-11416 | biostudies-arrayexpress

Transcription profiling of mouse HA specific T regulatory cells cultured in the presence of HA-pulsed dendritic cells and rhIL-2 with or without HA-specific T effector cells

Project description:Expanded HA-specific Thy-1.1 Tregs were cultured in the presence of HA-pulsed dendritic cells and rhIL-2 with or without HA-specific Teffs. After 5 days, Tregs were sorted by flow cytometry, based on the expression of the Thy-1.1 congeneic marker. Two biological duplicates were hybridized to the Sentrix BeadChips Array mouse WG-6 v2 (Illumina, USA). Data extraction and normalization was performed using Quantile normalization in the BeadStudio software.

2009-09-08 | E-MTAB-146 | biostudies-arrayexpress

ChIP-seq of Arabidopsis ARGONAUTE-small RNA interactions in Arabidopsis thaliana identified by high-throughput sequencing

Project description:The goal of this study was to identify small RNA-ARGONAUTE interactions in Arabidopsis. Co-immunoprecipitation assays followed by high-throughput sequencing were done to identify small RNA associated with HA-AGO2 and HA-AGO7. Experiment Overall Design: Sequencing-by-synthesis technology was used to sequence small RNA from input and co-immunoprecipitation fractions from cell lysates of inflorescence tissue from Arabidopsis plants transformed with HA epitope-tagged AGO2 or AGO7 constructs. Meaningful intepretation requires comparison between the input and co-immunoprecipitation datasets. Experiment Overall Design: Note: Raw data files requested, however, the submitter declined to provide them.

2009-08-30 | E-GEOD-12037 | biostudies-arrayexpress

ChIP-Seq MyT1-HA in NS5

Project description:Here we performed a ChIP-seq experiment on a sample of adherent cultures of mouse neural stem cells (NS5 cell line) expressing an inducible HA-tagged version of the proneural factor MyT1 (MyT1-HA, under TetON control) after activation by doxycycline hyclate (DOX). This resulted in the generation of a genome-wide map of MyT1-HA binding to chromatin.

2016-09-09 | E-MTAB-4330 | biostudies-arrayexpress

FYRN and FYRC domains of the histone demethylase, JMJ14, mediated chromatin binding and transgene silencing through a pair of NAC transcription factors

Project description:JmjC domain containing protein 14 (JMJ14) is an H3K4-specific histone demethylase that plays important roles in RNA-mediated gene silencing and flowering time regulation in Arabidopsis. However, how JMJ14 is recruited to their target genes remains unclear. Here, we show that the C-terminal FYRN and FYRC domains of JMJ14 are required for RNA silencing and flowering time regulation. Chromatin binding of JMJ14 is lost upon deletion of its FYRN and FYRC domains, along with increased H3K4me3. FYRN and FYRC domains interact with a pair of NAC domain containing transcription factors, NAC050 and NAC052. Genome-wide analysis revealed that JMJ14 and NAC050/052 share a set of common target genes with CTTGNNNNNCAAG consensus sequences. Mutations in either NAC052 or NAC050 impair RNA-mediated gene silencing. Thus, our findings demonstrate an important role of FYRN and FYRC in targeting JMJ14 through direct interaction with NAC050/052 transcription factors, which reveals a novel mechanism of recruiting a histone demethylases to its target loci in higher plants. anti-HA ChIP-seq were performed with six samples: Col, NAC050-HA, NAC052-HA, jmj14-1, JMJ14-HA and JMJ14-FYR-HA. Anti-H3K4me3 ChIP were performed with four samples: Col, jmj14-1, JMJ14-HA and JMJ14-FYR-HA. (NAC050-HA indicates PNAC050::NAC050-HA nac050-1, NAC052-HA indicates PNAC052::NAC052-HA nac052-2, JMJ14-HA indicates PJMJ14::JMJ14-HA jmj14-1, JMJ14-FYR-HA indicates PJMJ14::JMJ14-FYR-HA jmj14-1)

2015-08-28 | E-GEOD-60084 | biostudies-arrayexpress

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